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anti-ACAN antibody :: Mouse Aggrecan, N-terminal neoepitope FFGV Monoclonal Antibody

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Catalog # MBS442016
Unit / Price
  0.1 mg  /  $435 +1 FREE 8GB USB
Product Data
Product Name

Aggrecan, N-terminal neoepitope FFGV (ACAN), Monoclonal Antibody

Also Known As

Monocloncal Antibody FFGV (Clone BC14)

Product Synonym Names
Aggrecan Antibody, N-terminal neoepitope FFGV
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
721
Chromosome Location
Chromosome: 15; NC_000015.10 (88803442..88875354). Location: 15q26.1
OMIM
155760
3D Structure
ModBase 3D Structure for P16112
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
BC-14
Host
Mouse
Species Reactivity
Human, rat, guinea pig, horse, pig
Specificity
Recognizes the N-terminal neoepitope sequence (FFGV....) generated at the “MMP cleavage site” after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration).
Purity/Purification
Affinity purified on protein G
Form/Format
Liquid, 1 mL/vial
Concentration
0.1 mg/mL (lot specific)
Immunogen
Synthetic peptide: FFGVGGE
Myeloma
x63-Ag8.653
Light Chain Type
kappa
Technical Notes
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections as well as either alcohol-fixed frozen sections or un-fixed snap-frozen sections. Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-ACAN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACAN antibody
Aggrecan monoclonal antibody to N-terminal neoepitope FFGV (mouse, clone BC14). This fragment is rapidly released from the tissue when MMP catabolism of aggrecan occurs and has been identified in synovial fluid samples from patients with degenerative joint diseases.

Overview: Proteoglycans are categoried depending upon the nature of their glycosaminoglycan chains (chondroitin sulfate, dermatan sulfate, heparan sulphate and keratan sullfate) as well as characterized by size. Aggrecan is a large aggregating proteoglycan of articular cartilage. It is found also in aorta tissue, discs, tendons and in the perineuronal net. It is responsible for hydrating cartilage, giving it compressibility and resiliance during joint loading, thereby playing a major role in the normal function of cartilage. Depletion of glycosaminoglycan bearing aggrecan fragments is one of the earliest events in cartilage destruction. A) Aggrecan monomer showing proteolytic cleavage sites B) Aggregate showing aggrecan monomers bound to hyaluronic acid
Product Categories/Family for anti-ACAN antibody
Applications Tested/Suitable for anti-ACAN antibody
Western Blot (WB)
Application Notes for anti-ACAN antibody
Western-Blotting - Suggested dilution: 1:100. Detects a a band of approximately 60 - 150 kDa; ELISA; IHC

Product Data of anti-ACAN antibody
anti-ACAN antibody Product Data image
NCBI/Uniprot data below describe general gene information for ACAN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
250,193 Da[Similar Products]
NCBI Official Full Name
Aggrecan
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
NCBI Official Synonym Symbols
AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
  [Similar Products]
NCBI Protein Information
aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1
UniProt Protein Name
Aggrecan core protein
UniProt Synonym Protein Names
Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AGC1; CSPG1; MSK16; CSPCP  [Similar Products]
UniProt Entry Name
PGCA_HUMAN
NCBI Summary for ACAN
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACAN
Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Subunit structure: Interacts with FBLN1

By similarity. Interacts with COMP. Ref.11

Subcellular location: Secreted › extracellular space › extracellular matrix

By similarity.

Tissue specificity: Restricted to cartilages. Ref.4

Developmental stage: Expression was detected in chondrocytes throughout the developing skeleton.

Domain: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Post-translational modification: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

Involvement in disease: Spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]: Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9Spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN) [MIM:612813]: A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts.Note: The disease is caused by mutations affecting the gene represented in this entry.Osteochondritis dissecans short stature and early-onset osteoarthritis (OD) [MIM:165800]: A type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13

Sequence similarities: Belongs to the aggrecan/versican proteoglycan family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 4 Link domains.Contains 1 Sushi (CCP/SCR) domain.
Precautions
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Disclaimer
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Organs/Tissues associated with anti-ACAN antibody
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