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anti-C4B antibody :: Mouse Complement C4d Monoclonal Antibody

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Catalog # MBS605576
Unit / Price
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  0.1 mg  /  $730 +1 FREE 8GB USB
anti-C4B antibody
Product Name

Complement C4d (C4B), Monoclonal Antibody

Full Product Name

Complement C4d (Gg)

Product Synonym Names
Anti -Complement C4d (Gg)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (31982572..32003195). Location: 6p21.3
OMIM
120790
3D Structure
ModBase 3D Structure for P0C0L5
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G175
Host
Mouse
Species Reactivity
Guinea Pig, Human
Specificity
Recognizes an epitope on the alpha 2 chain of human C4d. It recognizes C4, C4b and C4d. Species crossreactivity: guinee pig
Purity/Purification
Affinity Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Immunogen
Human C4d.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-C4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-C4B antibody
Complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved resulting in C4c and C4d, in which step all functional sites are lost. Therefore, the C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.
Product Categories/Family for anti-C4B antibody
Applications Tested/Suitable for anti-C4B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-C4B antibody
Suitable for use in Western Blot, Immunohistochemistry (frozen), and Immunoassays.
Dilution: Western Blot: 1:50
Immunohistochemistry (frozen): 1:50
NCBI/Uniprot data below describe general gene information for C4B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
192,793 Da[Similar Products]
NCBI Official Full Name
C4 complement C4d region
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4B12; CPAMD3; FLJ60561; MGC164979
  [Similar Products]
NCBI Protein Information
complement C4-B; Chido form of C4; complement C4B1a; OTTHUMP00000029269; basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
UniProt Protein Name
Complement C4-B
UniProt Synonym Protein Names
Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CO4; CPAMD3  [Similar Products]
UniProt Entry Name
CO4B_HUMAN
NCBI Summary for C4B
This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9. [provided by RefSeq]
UniProt Comments for C4B
C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: complement binding; endopeptidase inhibitor activity; carbohydrate binding

Biological Process: detection of molecule of bacterial origin; regulation of complement activation; innate immune response; opsonization; inflammatory response; complement activation, classical pathway; complement activation

Disease: Complement Component 4b Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated with anti-C4B antibody
 Reference Product  PubMed Publications
 C4BPA antibody  >39 publications with C4B and C4BPA
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