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anti-BECN1 antibody :: Beclin 1 Monoclonal Antibody

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Catalog # MBS475123
Unit / Price
  0.1 mL  /  $335 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Beclin 1 (BECN1), Monoclonal Antibody

Popular Item
Full Product Name

Anti-Beclin 1 Mouse mAb

Product Synonym Names
APG6; ATG 6; ATG6; ATG6 autophagy related 6 homolog; Bcl-2-interacting protein beclin; Beclin 1 (coiled coil moesin like BCL2 interacting protein); Beclin 1 autophagy related; Beclin-1; Beclin1; BECN 1; BECN1; BECN1_HUMAN; Coiled coil myosin like BCL2 interacting protein; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS 30; VPS30
Product Synonym Gene Name
ATG6; VPS30; beclin1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF077301 mRNA
3D Structure
ModBase 3D Structure for Q14457
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4F4-B10-C9
Host
Mouse
Source: Human
Species Reactivity
Human, Mouse, Rat, Monkey, Hamster
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant human Beclin 1 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the beclin family.
Subunit Structure
Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 upregulates autophagy, while the one containing Rubicon downregulates autophagy By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB By similarity. Interacts with DAPK1.
Subcellular Location
Golgi apparatus; trans-Golgi network membrane; Peripheral membrane protein.
Other Notes
Small volumes of anti-BECN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BECN1 antibody
Entrez Summary: Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).
UniPort Summary Function: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
Applications Tested/Suitable for anti-BECN1 antibody
Western Blot (WB)
Application Notes for anti-BECN1 antibody
WB: 1:1000

Western Blot (WB) of anti-BECN1 antibody
Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using Beclin 1 mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.
anti-BECN1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for BECN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55
NCBI Official Full Name
beclin-1 isoform a
NCBI Official Synonym Full Names
beclin 1
NCBI Official Symbol
BECN1  [Similar Products]
NCBI Official Synonym Symbols
ATG6; VPS30; beclin1
  [Similar Products]
NCBI Protein Information
beclin-1
UniProt Protein Name
Beclin-1
UniProt Synonym Protein Names
Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Protein Family
UniProt Gene Name
BECN1  [Similar Products]
UniProt Synonym Gene Names
GT197  [Similar Products]
NCBI Summary for BECN1
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
UniProt Comments for BECN1
beclin 1: an essential autophagy protein that has been linked to multiple processes including tumor suppression, protection against some cardiac and neurological degenerative diseases, and lifespan extension. A ubiquitous protein of the beclin family. Is part of the class III PI3K complex that induces autophagy. Expressed at elevated levels in dendrites and cell bodies of cerebellar Purkinje cells. Interacts with the anti-apoptotic protein Bcl-2, which inhibits autophagy when it is present in the endoplasmic reticulum. The dissociation of ATG6 from Bcl-2 is essential for its autophagic activity. Interacts with PITC and GluR-delta2. Probably forms a complex with AMBRA1 and PIK3C3. Plays a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

Protein type: Adaptor/scaffold; Autophagy; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cytosol; endoplasmic reticulum; endosome; extrinsic to membrane; mitochondrion

Molecular Function: phosphoinositide 3-kinase binding; protein binding; ubiquitin protein ligase binding

Biological Process: autophagic vacuole formation; autophagy; cellular defense response; cellular response to glucose starvation; cellular response to nitrogen starvation; CVT pathway; cytokinesis; late endosome to vacuole transport; macroautophagy; mitotic metaphase plate congression; positive regulation of autophagy; receptor catabolic process; regulation of cytokinesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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