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anti-p53 antibody :: Mouse p53 (a.a. 371-380) Monoclonal Antibody

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Catalog # MBS395175
Unit / Price
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  0.1 mg  /  $340 +1 FREE 8GB USB
anti-p53 antibody
Product Name

p53 (a.a. 371-380), Monoclonal Antibody

Popular Item
Full Product Name

p53 (a.a. 371-380)

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000076.6 (61715263..61739260). Location: 10 B4; 10
3D Structure
ModBase 3D Structure for Q8BUE4
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
Pab122
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Monkey, Hamster
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Immunogen
Hybridoma produced by the fusion of splenocytes from immunized with full length mouse p53 protein and mouse myeloma cells.
Positive Control
Breast or colon carcinoma
Comment
THis antibody can be used for Western Blotting (5-10ug/ml, immunohistochemistry on frozen and formalin/paraffain embedded tissues (1-2ug/ml) and immunoprecipitation (2ug/mg of protein lysate). Optimal concentration should be evaluated by serial dilutions.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Shipping: Ship at ambient temperature, freeze upton arrival.
Stability: Products are stable for one year from puchase when stored properly.
Other Notes
Small volumes of anti-p53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-p53 antibody
p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
Applications Tested/Suitable for anti-p53 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
NCBI/Uniprot data below describe general gene information for p53. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,635 Da
NCBI Official Full Name
apoptosis-inducing factor 2 isoform 2
NCBI Official Synonym Full Names
apoptosis-inducing factor, mitochondrion-associated 2
NCBI Official Symbol
Aifm2  [Similar Products]
NCBI Official Synonym Symbols
Amid; PRG3; 5430437E11Rik; D730001I10Rik
  [Similar Products]
NCBI Protein Information
apoptosis-inducing factor 2; apoptosis-inducing factor-like mitchondrion-associated inducer of death; apoptosis-inducing factor-like mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor homologous mitochondrion-associated inducer of death
UniProt Protein Name
Apoptosis-inducing factor 2
UniProt Synonym Protein Names
Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death
UniProt Gene Name
Aifm2  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
AIFM2_MOUSE
UniProt Comments for p53
Function: Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death

By similarity. May contribute to genotoxin-induced growth arrest.

Cofactor: FAD. Binds 6-hydroxy-FAD non-covalently

By similarity.

Subcellular location: Cytoplasm. Mitochondrion outer membrane

By similarity.

Tissue specificity: Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in liver, testis, and kidney, and expressed at lower levels in pancreas, spleen, brain and lung. Ref.4

Induction: Expression is up-regulated in mouse embryonic fibroblasts by genotoxic reagents 5-fluorouracil and etoposide. Ref.4

Sequence similarities: Belongs to the FAD-dependent oxidoreductase family.

Sequence caution: Isoform 2: The sequence AAH38129.1 differs from that shown. Reason: erroneous termination (Translated as Trp in position: 339:Stop->W)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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