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anti-ORC1 antibody :: Rat ORC1 Monoclonal Antibody

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Catalog # MBS602347
Unit / Price
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  0.1 mL  /  $550 +1 FREE 8GB USB
anti-ORC1 antibody
Product Name

ORC1, Monoclonal Antibody

Full Product Name

ORC1 (Origin Recognition Complex Protein subunit 1)

Product Synonym Names
Anti -ORC1 (Origin Recognition Complex Protein subunit 1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (52838501..52870143, complement). Location: 1p32
OMIM
224690
3D Structure
ModBase 3D Structure for Q13415
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H45
Host
Rat
Species Reactivity
Hamster, Human, Mouse, Rat
Specificity
Recognizes endogenous levels of total human ORC1 protein. Does not crossreact with other ORC subunits. Species Crossreactivity: Hamster, mouse and rat.
Purity/Purification
Ascites
Ascites
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Immunogen
His6-tagged recombinant human ORC1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-ORC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ORC1 antibody
The origin recognition complex (ORC) is a highly conserved heterohexameric protein complex that associates with DNA at or near initiation of DNA replication sites. All six ORC subunits are essential for initiation of DNA replication (1-3), and ORC may be involved in regulation of gene expression in response to stress (4). ORC binding to DNA permits the ordered binding of other proteins such as cdc6 and MCMs to form pre-replication complexes (Pre-RCs). Pre-RCs form between telophase and early G1 phase of the cell cycle and are inactivated at the onset of DNA synthesis, allowing coordinated regulation of DNA replication and cell division (5). Modification of one or more of the six ORC subunits may be responsible for its inactivation during S phase, but the chromatin binding behavior of the ORC subunits during the cell division cycle is still under investigation (6-7).
Applications Tested/Suitable for anti-ORC1 antibody
Western Blot (WB)
Application Notes for anti-ORC1 antibody
Suitable for use in Western Blot.
NCBI/Uniprot data below describe general gene information for ORC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,350 Da[Similar Products]
NCBI Official Full Name
origin recognition complex subunit 1 isoform 1
NCBI Official Synonym Full Names
origin recognition complex, subunit 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ORC1L; PARC1; HSORC1
  [Similar Products]
NCBI Protein Information
origin recognition complex subunit 1; OTTHUMP00000009797; OTTHUMP00000009798; replication control protein 1; origin recognition complex, subunit 1-like; origin recognition complex, subunit 1 homolog
UniProt Protein Name
Origin recognition complex subunit 1
UniProt Synonym Protein Names
Replication control protein 1
UniProt Gene Name
UniProt Synonym Gene Names
ORC1L; PARC1  [Similar Products]
UniProt Entry Name
ORC1_HUMAN
NCBI Summary for ORC1
The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for ORC1
ORC1L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with CDC6 and KAT7/HBO1. Belongs to the ORC1 family.

Protein type: DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleolus; nuclear origin of replication recognition complex; cytosol; nucleus; origin recognition complex

Molecular Function: protein binding; DNA binding; chromatin binding; ATP binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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