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anti-CD40 antibody :: Mouse CD40 Monoclonal Antibody

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Catalog # MBS9200224
Unit / Price
  0.1 mL  /  $340 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CD40, Monoclonal Antibody

Popular Item
Also Known As

CD40 Antibody (C-term) (Ascites)

Product Synonym Names
Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; CD40; TNFRSF5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
247-276
Immunogen Sequence Length
277
OMIM
109535
3D Structure
ModBase 3D Structure for P25942
Clonality
Monoclonal
Isotype
IgG1
Clone Number
647CT13.2.4
Host
Mouse
Species Reactivity
Human (Predicted Reactivity: Horse, Mouse, Pig)
Specificity
This CD40 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the C-terminal region of human CD40.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Calculated MW
30619
Antigen Region
247-276
Antigen Source
HUMAN
Function
Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Cellular Location
Isoform I: Cell membrane; Single-pass type I membrane protein
Tissue Location
B-cells and in primary carcinomas.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-CD40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD40 antibody
The protein encoded by this gene is a member of the
TNF-receptor superfamily. This receptor has been found to be
essential in mediating a broad variety of immune and inflammatory
responses including T cell-dependent immunoglobulin class
switching, memory B cell development, and germinal center
formation. AT-hook transcription factor AKNA is reported to
coordinately regulate the expression of this receptor and its
ligand, which may be important for homotypic cell interactions.
Adaptor protein TNFR2 interacts with this receptor and serves as a
mediator of the signal transduction. The interaction of this
receptor and its ligand is found to be necessary for
amyloid-beta-induced microglial activation, and thus is thought to
be an early event in Alzheimer disease pathogenesis. Two
alternatively spliced transcript variants of this gene encoding
distinct isoforms have been reported.
Applications Tested/Suitable for anti-CD40 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD40 antibody
WB~~1:100~1600

Western Blot (WB) of anti-CD40 antibody
CD40 Antibody (C-term)(Ascites) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CD40 antibody detected the CD40 protein (arrow).
anti-CD40 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CD40. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human (Predicted Reactivity: Horse, Mouse, Pig)NP_690593.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
  [Similar Products]
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Synonym Protein Names
B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD_antigen: CD40
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TNFRSF5  [Similar Products]
UniProt Entry Name
TNR5_HUMAN
NCBI Summary for CD40
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
UniProt Comments for CD40
CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; signal transducer activity; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; platelet activation; positive regulation of isotype switching to IgG isotypes; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of B cell proliferation; protein kinase B signaling cascade; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion

Disease: Immunodeficiency With Hyper-igm, Type 3
Product References and Citations for anti-CD40 antibody
Rodriguez-Rodriguez, L., et al. J. Rheumatol. 37(10):2076-2080(2010)
Lewis, J.P., et al. Genomics 96(4):211-219(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Blanco-Kelly, F., et al. PLoS ONE 5 (7), E11520 (2010) :
Soliman, M.A., et al. Egypt J Immunol 16(1):61-70(2009)

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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