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anti-TNFAIP3 antibody :: Mouse anti-Human A-20/TNFAIP3 Monoclonal Antibody

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Catalog # MBS668220
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

A-20/TNFAIP3, Monoclonal Antibody

Popular Item
Full Product Name

A-20/TNFAIP3,Monoclonal antibody to A-20/TNFAIP3 (Clone: ABM1G20)

Product Synonym Names
TNFAIP3, OTUD7C
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
191163
3D Structure
ModBase 3D Structure for P21580
Clonality
Monoclonal
Isotype
IgG1, Kappa
Clone Number
ABM1G20
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein G choromatography
Form/Format
Purified
25 ug in 50 uL/ 100 ug in 200 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Immunogen
A partial length recombinant protein from C terminal portion of A20 was used as the immunogen for this antibody.
Buffer
PBS containing 0.05% BSA and 0.05% sodium azide.
Target Modification
No
Produced from Ascites
No
Preparation and Storage
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of anti-TNFAIP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TNFAIP3 antibody
Monoclonal antibody to A-20/TNFAIP3 (Clone: ABM1G20)
Applications Tested/Suitable for anti-TNFAIP3 antibody
FACS, WB
Application Notes for anti-TNFAIP3 antibody
Western Blot: 4-6 ug/mL

Western Blot (WB) of anti-TNFAIP3 antibody
Expression analysis of A20. Anti-A20 antibody (Clone: ABM1G20) was tested at 4 ug/ml on THP-1 Lysate.
anti-TNFAIP3 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC) of anti-TNFAIP3 antibody
Cell surface FLOW analysis of A20 (Clone : ABM1G20) on HepG2 cells using 0.5 ug/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control , red represents anti-A20 antibody
anti-TNFAIP3 antibody Flow Cytometry (FC) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for TNFAIP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 3
NCBI Official Synonym Full Names
TNF alpha induced protein 3
NCBI Official Symbol
TNFAIP3  [Similar Products]
NCBI Official Synonym Symbols
A20; AISBL; OTUD7C; TNFA1P2
  [Similar Products]
NCBI Protein Information
tumor necrosis factor alpha-induced protein 3
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 3
UniProt Synonym Protein Names
OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20
UniProt Gene Name
TNFAIP3  [Similar Products]
UniProt Synonym Gene Names
OTUD7C; TNF alpha-induced protein 3  [Similar Products]
UniProt Entry Name
TNAP3_HUMAN
NCBI Summary for TNFAIP3
This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Comments for TNFAIP3
TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.

Protein type: Apoptosis; Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; cytosol; lysosome; nucleus

Molecular Function: DNA binding; kinase binding; ligase activity; protease binding; protein binding; protein self-association; ubiquitin binding; ubiquitin-protein ligase activity; ubiquitin-specific protease activity; zinc ion binding

Biological Process: apoptosis; B-1 B cell homeostasis; inflammatory response; inhibition of NF-kappaB transcription factor; negative regulation of B cell activation; negative regulation of bone resorption; negative regulation of chronic inflammatory response; negative regulation of cyclin-dependent protein kinase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of inflammatory response; negative regulation of innate immune response; negative regulation of interferon type I production; negative regulation of interleukin-1 beta production; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; negative regulation of protein ubiquitination; negative regulation of smooth muscle cell proliferation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; negative regulation of toll-like receptor 5 signaling pathway; negative regulation of tumor necrosis factor production; positive regulation of protein catabolic process; protein deubiquitination; protein oligomerization; regulation of defense response to virus by host; regulation of germinal center formation; regulation of inflammatory response; response to molecule of bacterial origin; response to muramyl dipeptide
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Disclaimer
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