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anti-ADAMTS4 antibody :: Mouse anti-Human A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4) Monoclonal Antibody

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Catalog # MBS2001335 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $185 +1 FREE 8GB USB
  0.2 mg  /  $235 +1 FREE 8GB USB
  1 mg  /  $540 +1 FREE 8GB USB
Western Blot (WB)
Product Name

A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4), Monoclonal Antibody

Popular Item
Also Known As

Monoclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 4 (ADAMTS4)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2001335 Technical Datasheet
Immunogen Sequence Length
837
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-TFPG KDYDADRQCQ LTFGPDSRHC PQLPPPCAAL WCSGHLNGHA MCQTKHSPWA DGTPCGPAQA CMGGRCLHMD QLQDFNIPQA GGWGPWGPWG
OMIM
AF148213 mRNA
3D Structure
ModBase 3D Structure for O75173
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
1#
Host
Mouse
Species Reactivity
Human
Specificity
The antibody is a mouse monoclonal antibody raised against ADAMTS4. It has been selected for its ability to recognize ADAMTS4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
Concentration
1mg/ml (lot specific)
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ADAMTS4 (Thr437~Pro575 (Accession # O75173)) expressed in E.coli
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-ADAMTS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ADAMTS4 antibody
WB; IHC; ICC; IP.
Application Notes for anti-ADAMTS4 antibody
Western blotting: 0.5-2 Ug/mL;1:500-2000
Immunohistochemistry: 5-20 Ug/mL;1:50-200
Immunocytochemistry: 5-20 Ug/mL;1:50-200

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-ADAMTS4 antibody
Western Blot: Sample: Recombinant ADAMTS4, Human.
anti-ADAMTS4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ADAMTS4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90,065 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 4 isoform 2
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 4
NCBI Official Symbol
ADAMTS4  [Similar Products]
NCBI Official Synonym Symbols
ADMP-1; ADAMTS-2; ADAMTS-4
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 4
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 4
UniProt Synonym Protein Names
ADMP-1; Aggrecanase-1
UniProt Gene Name
ADAMTS4  [Similar Products]
UniProt Synonym Gene Names
KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4  [Similar Products]
NCBI Summary for ADAMTS4
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The expression of this gene is upregulated in arthritic disease and this may contribute to disease progression through the degradation of aggrecan. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
UniProt Comments for ADAMTS4
ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: EC 3.4.24.82; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: extracellular matrix; extracellular region; extracellular space; nuclear speck; proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protease binding; protein binding; zinc ion binding

Biological Process: extracellular matrix disassembly; proteolysis; skeletal system development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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