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anti-A-RAF antibody :: Rabbit anti-Human A-RAF Monoclonal Antibody

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Catalog # MBS179793
Unit / Price
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  0.1 mg  /  $340 +1 FREE 8GB USB
Western Blot (WB)
Product Name

A-RAF, Monoclonal Antibody

Full Product Name

Anti-A-RAF Rabbit Monoclonal Antibody

Product Synonym Names
Serine/threonine-protein kinase A-Raf; 2.7.11.1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X04790 mRNA
3D Structure
ModBase 3D Structure for P10398
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Immunogen
A synthesized peptide derived from human A-RAF
Tissue Specificity
Predominantly in urogenital tissues.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-A-RAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-A-RAF antibody
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Applications Tested/Suitable for anti-A-RAF antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-A-RAF antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-A-RAF antibody
Western blot analysis of A-RAF expression in A375 cell lysate (MBS179793).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARAF monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARAF
anti-A-RAF antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for A-RAF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67585 MW
NCBI Official Full Name
serine/threonine-protein kinase A-Raf isoform 2
NCBI Official Synonym Full Names
A-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
PKS2; A-RAF; ARAF1; RAFA1
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase A-Raf
UniProt Protein Name
Serine/threonine-protein kinase A-Raf
UniProt Synonym Protein Names
Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks
UniProt Gene Name
UniProt Synonym Gene Names
ARAF1; PKS; PKS2  [Similar Products]
NCBI Summary for A-RAF
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
UniProt Comments for A-RAF
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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