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anti-SERPINA1 antibody :: Mouse anti-Human a1-Antitrypsin Monoclonal Antibody

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Catalog # MBS604011
Unit / Price
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  0.1 mL  /  $525 +1 FREE 8GB USB
anti-SERPINA1 antibody
Product Name

a1-Antitrypsin (SERPINA1), Monoclonal Antibody

Popular Item
Full Product Name

a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI, native and denatured)

Product Synonym Names
Anti -a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI, native and denatured)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_000014.8 (94843084..94857029, complement). Location: 14q32.1
OMIM
107400
3D Structure
ModBase 3D Structure for P01009
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6F5
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human a-1 AT, native and denatured. Epitope specificity differs from that of clone 6F4. Reacts strongly with a-1 AT. Strong reaction is seen in ELISA with native and denatured a-1 AT directly coated onto the microtiter well. When tested in sandwich ELISA in combination with a polyclonal antibody against a-1 AT, it only recognizes native a-1 AT. In Western Blot after SDS-PAGE, this antibody reacts with native and denatured a-1 AT in reduced as well as non-reduced forms.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
Immunogen
a-1 AT isolated from human plasma.
Culture Medium
RPMI 1640 with 2-10% fatal calf serum
Hybridoma
X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-SERPINA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SERPINA1 antibody
The serum glycoprotein a1-antitrypsin (a-1 AT) is a typical member of the serpine family of serine protease inhibitors, and an inhibitor of the enzyme trypsin. a-1 AT is synthesized in the liver as a single polypeptide chain of approximately 51kD showing a marked degree of genetic polymorphism due to the presence of over 20 different alleles. Individuals homozygous for a-1 AT deficiency are predisposed to pulmonary emphysema and juvenile hepatic cirrhosis because of alterations in the amino acid and sialic acid components of the glycoprotein (1).
Applications Tested/Suitable for anti-SERPINA1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SERPINA1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:7500
NCBI/Uniprot data below describe general gene information for SERPINA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,737 Da[Similar Products]
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol
SERPINA1  [Similar Products]
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
  [Similar Products]
NCBI Protein Information
alpha-1-antitrypsin; serpin A1; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Synonym Protein Names
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1
Protein Family
UniProt Gene Name
SERPINA1  [Similar Products]
UniProt Synonym Gene Names
AAT; PI; SPAAT  [Similar Products]
UniProt Entry Name
A1AT_HUMAN
NCBI Summary for SERPINA1
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SERPINA1
SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; glycoprotein binding

Biological Process: platelet activation; response to chromate; platelet degranulation; response to triglyceride; response to cytokine stimulus; response to lead ion; acute-phase response; response to hypoxia; response to methanol; response to lipopolysaccharide; blood coagulation; response to estradiol stimulus

Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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