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anti-A1 antibody :: Mouse anti-Human A1 Monoclonal Antibody

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Catalog # MBS200362
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

A1, Monoclonal Antibody

Full Product Name

A1 antibody

Product Synonym Names
Annexin-1; Lipocortin I; Calpactin II; Chromobindin-9; p35; ANXA1; ANX1; LPC1; Annexin I; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANXA 1; ANXA1 protein; CalpactinII; Chromobindin 9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; Phospholipase A2 inhibitory protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X05908 mRNA
3D Structure
ModBase 3D Structure for P04083
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2G5
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human Annexin A1 (1-346aa) purified from E Coli.
Clone Note
Anti-human Annexin A1 mAb, clone AT2G5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Annexin A1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-A1 antibody
Annexin A1, also known as Lipocortin I, belongs to a family of Ca (2+)-dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. It promotes membrane fusion and is also involved in exocytosis. Since Annexin A1 has phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity, this protein may have potential anti-inflammatory activity. The detection of this protein by immunocytochemical means reportedly provides a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.
Applications Tested/Suitable for anti-A1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-A1 antibody
Recommended starting dilution is 1:3000-1:20000.

Western Blot (WB) of anti-A1 antibody
Western blot analysis The Recombinant Human Annexin A1 (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Annexin A1 Lane 2.: HeLa cell lysate Lane 3.: A549 cell lysate Lane 4.: A431 cell lysate The HeLa cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Anti- Annexin A1 antibody (1:10000) Lane 2.: Anti- Annexin A1 antibody (1:20000)
anti-A1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for A1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
38,714 Da
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
ANXA1  [Similar Products]
NCBI Official Synonym Symbols
ANX1; LPC1
  [Similar Products]
NCBI Protein Information
annexin A1
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I
Protein Family
UniProt Gene Name
ANXA1  [Similar Products]
UniProt Synonym Gene Names
ANX1; LPC1  [Similar Products]
NCBI Summary for A1
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
UniProt Comments for A1
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca2+ and can bind up to eight Ca2+ ions (). Displays Ca2+-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton ().
Product References and Citations for anti-A1 antibody
Lim L.H., et al. (2007) FASEB J. 21 (4): 968-975; Kovacic R.T., et al. (1991) Biochemistry. 30 (37): 9015-9021

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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