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anti-ACTB antibody :: Mouse anti-Human ACTB Monoclonal Antibody

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Catalog # MBS120059
Unit / Price
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  0.1 mg  /  $395 +1 FREE 8GB USB
Product Name

ACTB, Monoclonal Antibody

Popular Item
Full Product Name

Mouse monoclonal antibody Anti-Human ACTB

Product Synonym Names
Homo sapiens actin, beta; PS1TP5BP1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
102630
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ACTBD11B7
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTB antibody
Beta actin is one of six different actin isoforms which have been identified. ACTB is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-ACTB antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-ACTB antibody
Western blot analysis of immunized recombinant protein, using anti-ACTB monoclonal antibody.
anti-ACTB antibody Quality Control image
Quality Control #2 of anti-ACTB antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-ACTB antibody Quality Control #2 image
Western Blot (WB) of anti-ACTB antibody
Detection of ACTB by Western blot.

Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. ACTBD11B7-2]

Predicted molecular weight: 41 kDa
anti-ACTB antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-ACTB antibody
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ACTB mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. ACTBD11B7-1]
anti-ACTB antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunocytochemistry (ICC) of anti-ACTB antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ACTB mAb. [Lot No. ACTBD11B7-1]
anti-ACTB antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,737 Da
NCBI Official Full Name
Homo sapiens actin, beta, mRNA
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
  [Similar Products]
NCBI Protein Information
actin, cytoplasmic 1; PS1TP5-binding protein 1; beta cytoskeletal actin
Protein Family
UniProt Gene Name
UniProt Entry Name
ACTB_HUMAN
NCBI Summary for ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
UniProt Comments for ACTB
ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; ribonucleoprotein complex; cytosol; nucleoplasm; cytoskeleton; membrane; axon; nuclear chromatin; cytoplasm; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; 'de novo' posttranslational protein folding; retinal homeostasis; cellular protein metabolic process; substantia nigra development; ATP-dependent chromatin remodeling; ephrin receptor signaling pathway; innate immune response; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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