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anti-ACTB antibody :: Mouse anti-Human ACTB Monoclonal Antibody

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Catalog # MBS200463
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

ACTB, Monoclonal Antibody

Full Product Name

ACTB antibody

Product Synonym Names
Actin; cytoplasmic 1; Beta-actin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X00351 mRNA
3D Structure
ModBase 3D Structure for P60709
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT4G6
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human ACTB (1-375aa) purified from E. coli.
Clone Note
Anti-human ACTB mAb, clone AT4G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTB protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACTB antibody
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Product Categories/Family for anti-ACTB antibody
Applications Tested/Suitable for anti-ACTB antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-ACTB antibody
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: PC3 cell lysate Lane 5.: A431 cell lysate Lane 6.: HepG2 cell lysate Lane 7.: MCF7 cell lysate Lane 8.: U87-MG cell lysate Lane 9.: NIH-3T3 cell lysate The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate Lane 2.: Kidney tissue lysate Lane 3.: Liver tissue lysate Lane 4.: Lung tissue lysate Lane 5.: Spleen tissue lysate Lane 6.: Testis tissue lysate Lane 7.: Bone marrow tissue lysate Lane 8.: Stomach tissue lysate The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: mouse monoclonal ACTB antibody 1:1000 Lane 2.: mouse monoclonal ACTB antibody 1:3000 Lane 3.: mouse monoclonal ACTB antibody 1:5000 Lane 4.: mouse monoclonal ACTB antibody 1:10000
anti-ACTB antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ACTB antibody
ICC/IF analysis of ACTB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
anti-ACTB antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,737 Da
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
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NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
NCBI Summary for ACTB
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
UniProt Comments for ACTB
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Product References and Citations for anti-ACTB antibody
Gunning PW., et al. (2015) Journal of Cell Science. 128 (11): 2009-2019.; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4): 673-8.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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