• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-ADAM15 antibody :: Mouse anti-Human ADAM15 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS607344
Unit / Price
  0.1 mg  /  $680 +1 FREE 8GB USB
  0.5 mg  /  $1,350 +1 FREE 8GB USB
anti-ADAM15 antibody
Product Name

ADAM15, Monoclonal Antibody

Full Product Name

ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

Product Synonym Names
Anti -ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 1; Location: 1q21.3
OMIM
605548
3D Structure
ModBase 3D Structure for Q13444
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9H33
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human ADAM15 ectodomain.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Immunogen
Recombinant protein corresponding to aa207-696 from human ADAM15 ectodomain fused to the IgG Fc domain expressed in COS-7 cells. (Accession #Q13444)
Positive Control
MCF-7 cells
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-ADAM15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADAM15 antibody
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
Applications Tested/Suitable for anti-ADAM15 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-ADAM15 antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction 200ul.
Immunocytochemistry: 10ug/ml. Cells fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. After blocking, cells were incubated with diluted A0859-31B followed by labeled anti-mouse IgG.
Western Blot: 1-2ug/ml. Detects ~5ng/lane of rhADAM15 under non-reducing conditions.
Immunoprecipitation: 25ug/ml
Direct ELISA: 1ug/ml detects ~0.3ng rhADAM15/well.
NCBI/Uniprot data below describe general gene information for ADAM15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,959 Da[Similar Products]
NCBI Official Full Name
ADAM metallopeptidase domain 15
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 15
NCBI Official Symbol
ADAM15  [Similar Products]
NCBI Official Synonym Symbols
MDC15; ADAM15
  [Similar Products]
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 15; MDC-15; ADAM 15; metargidin; OTTHUMP00000033236; metalloprotease RGD disintegrin protein; a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 15
UniProt Synonym Protein Names
Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin
UniProt Gene Name
ADAM15  [Similar Products]
UniProt Synonym Gene Names
MDC15  [Similar Products]
UniProt Entry Name
ADA15_HUMAN
NCBI Summary for ADAM15
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
UniProt Comments for ADAM15
ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell projection; adherens junction; cell surface; acrosome; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; proteolysis; negative regulation of cell-matrix adhesion; collagen catabolic process; extracellular matrix disassembly; tissue regeneration; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000