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anti-ADAMTS4 antibody :: Mouse anti-Human ADAMTS4 Monoclonal Antibody

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Catalog # MBS608876
Unit / Price
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  0.5 mg  /  $810 +1 FREE 8GB USB
anti-ADAMTS4 antibody
Product Name

ADAMTS4, Monoclonal Antibody

Popular Item
Full Product Name

ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)

Product Synonym Names
Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (161159538..161168845, complement). Location: 1q21-q23
OMIM
603876
3D Structure
ModBase 3D Structure for O75173
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9H40
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human ADAMTS4. Does not crossreact with recombinant human ADAMTS1, ADAMTS5, ADAMTS13 or ADAMTSL1.2. Species Sequence Homology: chimpanzee; 100%, canine; 97%, mouse, rat, bovine; 94%
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Immunogen
Recombinant corresponding to aa213-685 from human ADAMTS4 expressed in NSO cells (O75173).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Other Notes
Small volumes of anti-ADAMTS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ADAMTS4 antibody
ADAMTS4 is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal peptide (aa1-51), a pro domain (aa52-212) and a mature chain (aa213-837) containing the following domains: catalytic (aa218-425), disintegrin (aa437-519), TSP type-1 (aa520-575), Cys-rich (aa577-685) and spacer (aa686-837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.
Applications Tested/Suitable for anti-ADAMTS4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Application Notes for anti-ADAMTS4 antibody
Suitable for use in Neutralization, Direct ELISA, Western Blot and Immunoprecipitation.
Dilution: Neutralization: 25ug/ml (IC50) inhibits 50% of the proteolytic activity under conditions in which the enzyme was present at 5ug/ml and the substrate concentration was 100ug/ml. The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with recombinant human Aggrecan G1-IGD-G2 domains as the substrate. Considering the molecular mass of the enzyme (50kD) and the antibody (150kD), IC50 was achieved at ~2:1 molar ratio of the antibody to enzyme.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human ADAMTS4 from conditioned cell medium.
NCBI/Uniprot data below describe general gene information for ADAMTS4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90,197 Da[Similar Products]
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 4
NCBI Official Symbol
ADAMTS4  [Similar Products]
NCBI Official Synonym Symbols
ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688
  [Similar Products]
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; OTTHUMP00000032249; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 4
UniProt Synonym Protein Names
ADMP-1; Aggrecanase-1
UniProt Gene Name
ADAMTS4  [Similar Products]
UniProt Synonym Gene Names
KIAA0688  [Similar Products]
UniProt Entry Name
ATS4_HUMAN
NCBI Summary for ADAMTS4
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq]
UniProt Comments for ADAMTS4
ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: EC 3.4.24.82; Secreted; Motility/polarity/chemotaxis; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; protease binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-ADAMTS4 antibody
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