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anti-AFP antibody :: anti-Human Alpha Fetoprotein (AFP) Monoclonal Antibody

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Catalog # MBS311666
Unit / Price
  1 mg  /  $295 +1 FREE 8GB USB
anti-AFP antibody
Product Name

Alpha Fetoprotein (AFP), Monoclonal Antibody

Popular Item
Also Known As

MAb to Alpha Fetoprotein (AFP)

Product Synonym Names
MAb to AFP; Monoclonal Antibody to Human Alpha Fetoprotein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
609
Chromosome Location
chromosome: 4; Location: 4q11-q13
OMIM
104150
3D Structure
ModBase 3D Structure for P02771
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
057-11301
Host
Host: Mouse
Source: Ascites
Species Reactivity
Human
Specificity
Alpha Fetoprotein (AFP)
Human Alpha Fetoprotein (AFP)
Purity/Purification
>90% pure (SDS-PAGE). Protein A chromatography
Form/Format
Purified, Liquid
Concentration
4.71 mg/ml (OD280nm, E^0.1% = 1.4) (lot specific)
Immunogen
Human AFP
Affinity Constant
Not determined
Buffer
10mM Phosphate, pH 7.4 containing 150mM Sodium chloride
Preservative
0.1% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Short-term (up to 7 days) store at 2 to 8 degree C. Long term, aliquot and store at <-20 degree C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freeze/th
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AFP antibody
ELISA; Immunoassay Antibody Pairs
NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
68,678 Da[Similar Products]
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
NCBI Official Synonym Symbols
FETA; HPAFP; AFP
  [Similar Products]
NCBI Protein Information
alpha-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Synonym Protein Names
Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HPAFP  [Similar Products]
UniProt Entry Name
FETA_HUMAN
NCBI Summary for AFP
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
UniProt Comments for AFP
Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular location: Secreted.

Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Developmental stage: Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

Post-translational modification: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.

Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.
Research Articles on AFP
1. Assessment of Dkk3 and AFP may be useful in the diagnosis of hepatic tumors.
2. AFP promoter enhancer increased specific expression of the human sodium iodide symporter (hNIS) for targeted radioiodine therapy of hepatocellular carcinoma.
3. Alpha-fetoprotein was more sensitive than des-gamma carboxyprothrombin and lectin-bound alpha-fetoprotein in diagnosing early hepatocellular carcinoma.
4. Case Report: Alpha-fetoprotein (AFP)-producing ovarian tumor in an elderly woman.
5. The utility of the tumour markers carcinoembryonic antigen, cancer antigen 125, carbohydrate antigen 19-9, carbohydrate antigen 15-3, alpha-fetoprotein and human chorionic gonadotropin for the diagnosis solitary pulmonary nodules, was investigated.
6. Case Report: Alpha-fetoprotein expressing metastastic adenocarcinoma of the esophago-gastric junction responding favorably to capecitabine and oxaliplatin.
7. small amplitude undulations in AFP are associated with recurrence of germ cell neoplasm
8. Low Alphafetoprotein levels and increased CA 19-9 levels can be used as tumor markers in diagnosis of molar pregnancies in conjunction with clinical and sonographic findings and serum levels of human chorionic gonadotropin
9. Report des-gamma-carboxyprothrombin, alpha-fetoprotein and AFP-L3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma.
10. These data support the idea that ZHX2 contributes to AFP repression in the liver after birth and may also be involved in AFP reactivation in liver cancer.
11. In hepatocellular carcinoma, pre-transplant AFP levels >400 ng/ml were associated with higher tumor recurrence.
12. Serum samples were investigated for carcinoembryonic antigen (CEA), CA125 and MUC1, alpha-foetoprotein, neuron-specific enolase and CA19.9.
13. In patients with hepatocellular carcinoma, a high frequency of AFP-specific CD8(+) T cells directed against different epitopes suggest that AFP has a strong and broad immunogenicity.
14. Molecular background of AFP in liver cancer cells as revealed by global RNA expression analysis is reported.
15. results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via dysfunction of the p53/Bax/cytochrome c/caspase-3 signaling pathway in AFP
16. Elevated alpha-fetoprotein is associated with primary central nervous system germ cell tumors.
17. Results indicate that the poor prognosis associated with high alpha-fetoprotein in hepatocellular carcinoma is due to high cell proliferation, high angiogenesis, and low apoptosis.
18. targeting of AFP with siRNA could be a potential therapeutic approach for hepatoma
19. substitution of aa 70 of HCV-1b core region is an important predictor of elevated AFP in non-HCC (hepatocellular carcinoma) patients.
20. The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-AFP antibody
 Reference Product  PubMed Publications
 CCR5 antibody  >2 publications with AFP and CCR5
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