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anti-APOA1 antibody :: Mouse anti-Human APOA1 Monoclonal Antibody

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Catalog # MBS200214
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

APOA1, Monoclonal Antibody

Also Known As

APOA1 antibody

Product Synonym Names
APOA1; apolipoprotein A-I; Apolipoprotein A1; Apo-AI; ApoA-I
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
267
OMIM
J00098 Genomic DNA
3D Structure
ModBase 3D Structure for P02647
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1E12
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human APOA1 (25-267aa) purified from E. coli
Clone Note
Anti-human APOA1 mAb, clone AT1E12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human APOA1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-APOA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APOA1 antibody
Apolipoprotein A1 (APOA1) is the main protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in APOA1 are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. High level of APOA1 is regarded as protective against atherosclerosis and cardiovascular disease.
Product Categories/Family for anti-APOA1 antibody
Applications Tested/Suitable for anti-APOA1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-APOA1 antibody
WB: 1:500~1:5000.
Recommended staring dilution is 1:1000

Western Blot (WB) of anti-APOA1 antibody
The extracts of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APOA1 (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-APOA1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for APOA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,778 Da
NCBI Official Full Name
apolipoprotein A-I isoform 1 preproprotein
NCBI Official Synonym Full Names
apolipoprotein A1
NCBI Official Symbol
APOA1  [Similar Products]
NCBI Official Synonym Symbols
apo(a)
  [Similar Products]
NCBI Protein Information
apolipoprotein A-I
UniProt Protein Name
Apolipoprotein A-I
UniProt Synonym Protein Names
Apolipoprotein A1
Protein Family
UniProt Gene Name
APOA1  [Similar Products]
UniProt Synonym Gene Names
Apo-AI; ApoA-I; ProapoA-I  [Similar Products]
NCBI Summary for APOA1
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
UniProt Comments for APOA1
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Product References and Citations for anti-APOA1 antibody
Dastani Z, et al. (March 2006). Atherosclerosis 185 (1): 127-36.; Chiesa G, Sirtori CR (2003). Curr. Opin. Lipidol. 14 (2): 159-63.; Pussinen, et al. (Jan. 1998). J. Lipid Res. (UNITED STATES) 39 (1): 152-61.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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