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anti-APOA1 antibody :: anti-Human Apolipoprotein AI (Apo AI) Monoclonal Antibody

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Catalog # MBS311600
Unit / Price
  1 mg  /  $305 +1 FREE 8GB USB
anti-APOA1 antibody
Product Name

Apolipoprotein AI (Apo AI) (APOA1), Monoclonal Antibody

Also Known As

MAb to Apolipoprotein AI

Product Synonym Names
Monoclonal Antibody to Human Apolipoprotein AI (Apo AI)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
267
Chromosome Location
Chromosome: 11; NC_000011.9 (116706469..116708338, complement). Location: 11q23-q24
OMIM
105200
3D Structure
ModBase 3D Structure for P02647
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
057-10029
Host
Host: Mouse
Source: Ascites
Species Reactivity
Human
Specificity
Apolipoprotein AI (Apo AI)
This product does not cross react with Apo AII or Apo B in competitive RIA.
Purity/Purification
>90% pure (SDS-PAGE). Protein A chromatography
Product is 0.2um filtered.
Form/Format
Purified, Liquid
Concentration
5.3mg/ml (OD280nm, E^0.1% = 1.4) (lot specific)
Immunogen
Human high density lipoprotein
Affinity Constant
5 x 10^6 L/m (determined by RIA followed by Scatchard plot analysis)
Buffer
10mM Phosphate, pH 7.4 containing 150mM Sodium chloride
Preservative
0.1% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Short term (up to 7 days) store at 2 to 8 degree C. Long term, aliquot and store at <-40 degree C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freeze/th
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-APOA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for APOA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
30,778 Da[Similar Products]
NCBI Official Full Name
apolipoprotein AI
NCBI Official Synonym Full Names
apolipoprotein A-I
NCBI Official Symbol
APOA1  [Similar Products]
NCBI Official Synonym Symbols
MGC117399
  [Similar Products]
NCBI Protein Information
apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
UniProt Protein Name
Apolipoprotein A-I
UniProt Synonym Protein Names
Apolipoprotein A1
Protein Family
UniProt Gene Name
APOA1  [Similar Products]
UniProt Entry Name
APOA1_HUMAN
NCBI Summary for APOA1
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]
UniProt Comments for APOA1
Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Ref.25 Ref.31

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. Ref.33

Post-translational modification: Palmitoylated. Ref.28Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [

MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant. Ref.37 Ref.38Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [

MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I. Ref.37 Ref.38Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [

MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed. Ref.37 Ref.38 Ref.43 Ref.44Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [

MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Ref.37 Ref.38 Ref.46

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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