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anti-APOB antibody :: Mouse anti-Human Apolipoprotein B (APOB) Monoclonal Antibody

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Catalog # MBS2090601
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $185 +1 FREE 8GB USB
  0.2 mg  /  $230 +1 FREE 8GB USB
  1 mg  /  $525 +1 FREE 8GB USB
anti-APOB antibody
Product Name

Apolipoprotein B (APOB), Monoclonal Antibody

Full Product Name

Monoclonal Antibody to Apolipoprotein B (APOB)

Product Synonym Names
Apo-B; FLDB; Apo B-100; Apo B-48
Matching Pairs
Unconjugated Antibody: Apolipoprotein B (APOB) Clone #C2 (MBS2090601)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
Matching Pairs
Unconjugated Antibody: Apolipoprotein B (APOB) Clone #C2 (MBS2090601)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
Matching Pairs
Unconjugated Antibody: Apolipoprotein B (APOB) Clone #C2 (MBS2090601)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X04506 mRNA
3D Structure
ModBase 3D Structure for P04114
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C2
Host
Mouse
Species Reactivity
Human
Specificity
The antibody is a mouse monoclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/mL (lot specific)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-APOB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-APOB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-APOB antibody
Western Blot: 0.5-5ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
515,605 Da
NCBI Official Full Name
apolipoprotein B-100
NCBI Official Synonym Full Names
apolipoprotein B
NCBI Official Symbol
NCBI Official Synonym Symbols
FLDB; LDLCQ4; apoB-48; apoB-100
  [Similar Products]
NCBI Protein Information
apolipoprotein B-100
UniProt Protein Name
Apolipoprotein B-100
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Apo B-100; Apo B-48  [Similar Products]
NCBI Summary for APOB
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
UniProt Comments for APOB
APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: cell soma; chylomicron; chylomicron remnant; cytoplasm; cytosol; early endosome; endocytic vesicle lumen; endoplasmic reticulum exit site; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular exosome; extracellular region; extracellular space; intermediate-density lipoprotein particle; intracellular membrane-bound organelle; low-density lipoprotein particle; lysosomal lumen; mature chylomicron; plasma membrane; smooth endoplasmic reticulum; very-low-density lipoprotein particle

Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding

Biological Process: artery morphogenesis; cellular protein metabolic process; cellular response to prostaglandin stimulus; cellular response to tumor necrosis factor; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; chylomicron assembly; chylomicron remnant clearance; chylomicron remodeling; fertilization; in utero embryonic development; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein transport; low-density lipoprotein particle clearance; low-density lipoprotein particle remodeling; nervous system development; positive regulation of cholesterol storage; positive regulation of gene expression; positive regulation of lipid storage; positive regulation of macrophage derived foam cell differentiation; post-embryonic development; post-translational protein modification; receptor-mediated endocytosis; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to estradiol; response to lipopolysaccharide; response to selenium ion; response to virus; retinoid metabolic process; sperm motility; spermatogenesis; toll-like receptor signaling pathway; triacylglycerol mobilization; triglyceride catabolic process; very-low-density lipoprotein particle assembly

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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