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anti-ATF6 antibody :: Mouse anti-Human ATF6 Monoclonal Antibody

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Catalog # MBS668021
Unit / Price
  0.1 mg  /  $290 +1 FREE 8GB USB
Product Name

ATF6, Monoclonal Antibody

Popular Item
Also Known As

ATF6 Monoclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
670
OMIM
605537
3D Structure
ModBase 3D Structure for P18850
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein G Chromatography
Form/Format
Purified
25 ug in 50 uL PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Immunogen
A partial recombinant ATF6 protein (amino acids 1-400) was used as the immunogen for this antibody
Preparation and Storage
Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of anti-ATF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATF6 antibody
ATF6, also named as Cyclic AMP-dependent transcription factor ATF-6 alpha is a member of bZIP family, ATF subfamily. ATF6 forms homodimer and heterodimer with ATF6-beta. ATF6 acts by activating specific unfolded protein response target genes during endoplasmic reticulum stress. ATF6 is a ubiquitously expressed transcription factor located at endoplasmic reticulum (ER) membrane. During ER stress an approximately 50kDa fragment containing the N-terminal cytoplasmic domain is cleaved by proteolysis and is translocated into the nucleus. N-glycosylation may serve as a sensor for ER homeostasis. For the binding of ATF6 to ERSE, ESRE should first bind to another transcription factor called Nuclear Transcription Factor Y (NF-Y). ATF6 could also be involved in activation of transcription by interacting with serum response factor. ATF6 is a 74 kDa protein and is highly expressed in kidney, liver, colon and mammary gland.
Product Categories/Family for anti-ATF6 antibody
Applications Tested/Suitable for anti-ATF6 antibody
FACS, WB
Application Notes for anti-ATF6 antibody
Western Blot analysis: 2-4 ug/mL
FACS: 0.5 ug/106 cells

Western Blot (WB) of anti-ATF6 antibody
Expression analysis of ATF6. Anti- ATF6 antibody (Clone: ABM1A40) was used at 2 ug/ml on K562 lysate.
anti-ATF6 antibody Western Blot (WB) (WB) image
Flow Cytometry of anti-ATF6 antibody
Intracellular staining of full length ATF6 plasmid and blank plasmid transfected HEK293 cells. 0.5 ug of anti-ATF6 antibody was used. Green represents blank plasmid, Red represents ATF6 expression plasmid. Goat anti-mouse PE was used as secondary antibody.
anti-ATF6 antibody Flow Cytometry (FC/FACS) image
Western Blot of anti-ATF6 antibody
Expression analysis of ATF6. Anti- ATF6 antibody (Clone ABM1A40) was used at 2 ug/ml on (1) Blank plasmid transfected HEK293 cells, (2) Partial length ATF6 plasmid transfected HEK293 cells and (3) Fulll length ATF6 plasmid transfected HEK293 cells.
anti-ATF6 antibody Western Blot (WB) image
NCBI/Uniprot data below describe general gene information for ATF6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
cyclic AMP-dependent transcription factor ATF-6 alpha
NCBI Official Synonym Full Names
activating transcription factor 6
NCBI Official Symbol
NCBI Official Synonym Symbols
ATF6A
  [Similar Products]
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-6 alpha
UniProt Synonym Protein Names
Activating transcription factor 6 alpha; ATF6-alpha
UniProt Gene Name
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha  [Similar Products]
UniProt Entry Name
ATF6A_HUMAN
NCBI Summary for ATF6
This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]
UniProt Comments for ATF6
ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; protein folding; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction

Disease: Achromatopsia 7
Precautions
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Disclaimer
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