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anti-B7-H4 antibody :: Mouse anti-Human B7-H4 Monoclonal Antibody

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Catalog # MBS668282
Unit / Price
  0.1 mg  /  $260 +1 FREE 8GB USB
Product Name

B7-H4, Monoclonal Antibody

Popular Item
Also Known As

Monoclonal Antibody to B7-H4 (Clone: ABM53A6)

Product Synonym Names
V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; Monoclonal antibody to B7H4 (Clone: ABM53A6)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
282
OMIM
AY280972 mRNA
3D Structure
ModBase 3D Structure for Q7Z7D3
Clonality
Monoclonal
Isotype
IgG2a k
Clone Number
ABM53A6
Host
Mouse
Species Reactivity
Human
Purity/Purification
Purified
Protein G Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Immunogen Information
A partial length recombinant protein (a.a 23-220) of B7-H4 was used as the immunogen for this antibody.
Antigen
B7H4
Conjugate
Unconjugated
Preparation and Storage
Store the antibody at 4°C, stable for 6 months.
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.
Other Notes
Small volumes of anti-B7-H4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-B7-H4 antibody
B7-H4, a member of B7 family, is a transmembrane protein that has been shown to inhibit T cell responses and neutrophil expansion during bacterial infections. B7-H4 mRNA is widely distributed in mouse and human peripheral tissues and can be induced in monocytes, macrophages, and dendritic cells upon IL-6 and IL-10 stimulation. However, in a variety of tumor cells, B7-H4 is predominantly present in intracellular compartments with unknown mechanism and functions. Cell surface expression of B7-H4 protein is limited and shows an inducible pattern on hematopoietic cells. The overexpression of B7-H4 has been found in various types of human tumors, such as breast cancer, renal cell carcinoma (RCC), ovarian cancer, esophageal squamous cell carcinoma, gastric cancer, pancreatic cancer and melanoma etc, where its expression level is positively correlated with disease progression. By arresting cell cycle, B7-H4 ligation of T cells has a profound inhibitory effect on the growth, cytokine secretion, and development of cytotoxicity.
Product Categories/Family for anti-B7-H4 antibody
Applications Tested/Suitable for anti-B7-H4 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-B7-H4 antibody
FACS: 0.5-1 ug/10^6 cells
IHC: 5 ug/ml
WB: 2-4 ug/ml

Immunohistochemistry (IHC) of anti-B7-H4 antibody
Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml
anti-B7-H4 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-B7-H4 antibody
Fig-2: Cell surface Flow analysis of B7-H4 antibody in human PBMC (Monocytes) cells using 0.5 ?g/ 10^6 cells of anti-B7-H4 antibody (ABM53A6). Green represents isotype control;red represents anti-B7H4 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
anti-B7-H4 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Western Blot (WB) of anti-B7-H4 antibody
Fig-3: Western blot analysis of B7H4. Anti-B7H4 antibody (Clone: ABM53A6) was used at 4 ug/ml on human B7H4-FC fusion protein Lysate.
anti-B7-H4 antibody Western Blot (WB) (WB) image
Western Blot(WB)Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 g/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates. of anti-B7-H4 antibody
anti-B7-H4 antibody Western Blot(WB)Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 µg/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates. (WB) image
NCBI/Uniprot data below describe general gene information for B7-H4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
V-set domain-containing T-cell activation inhibitor 1
NCBI Official Synonym Full Names
V-set domain containing T-cell activation inhibitor 1
NCBI Official Symbol
VTCN1  [Similar Products]
NCBI Official Synonym Symbols
B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
  [Similar Products]
NCBI Protein Information
V-set domain-containing T-cell activation inhibitor 1
UniProt Protein Name
V-set domain-containing T-cell activation inhibitor 1
UniProt Synonym Protein Names
B7 homolog 4; B7-H4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x
UniProt Gene Name
VTCN1  [Similar Products]
UniProt Synonym Gene Names
B7-H4  [Similar Products]
NCBI Summary for B7-H4
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Comments for B7-H4
VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1-p12
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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