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anti-bcl-2 antibody :: Mouse anti-Human bcl-2 Monoclonal Antibody

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Catalog # MBS370007
Unit / Price
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  5 Control Slides  /  $130 +1 FREE 8GB USB
  0.1 mL (Concentrate)  /  $165 +1 FREE 8GB USB
  3 ml (Prediluted)  /  $165 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $190 +1 FREE 8GB USB
  0.5 mL (Concentrate)  /  $245 +1 FREE 8GB USB
  1 mL (Concentrate)  /  $300 +1 FREE 8GB USB
  15 mL (Prediluted)  /  $300 +1 FREE 8GB USB
Product Name

bcl-2, Monoclonal Antibody

Popular Item
Full Product Name

bcl-2

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS370007 Technical Datasheet
Chromosome Location
Chromosome: 13; NC_005112.2 (12730736..12905108, complement). Location: 13p13
3D Structure
ModBase 3D Structure for P49950
Clonality
Monoclonal
Isotype
IgG1/K
Clone Number
BCL2/A4
Host
Mouse
Species Reactivity
Human
Form/Format
Anti-bcl-2 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
Control
Tonsil, Lymph Node
Localization
Cytoplasmic
Reactivity Note
Paraffin, Frozen
Preparation and Storage
Store at 2 to 8 degree C in the dark.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of anti-bcl-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-bcl-2 antibody
bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of bcl-2, such as in the case of translocation of bcl-2 to Ig heavy-chain loci, is thought to be the cause of Follicular Lymphoma. Anti-bcl-2 has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in Follicular Lymphoma. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This antibody may also be used in distinguishing between those Follicular Lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2-negative. Anti-bcl-2 has been used as a predictive biomarker for recurrence of Cancer of the Breast and Non-Small-Cell Carcinoma of the Lung.

Dilution Information of anti-bcl-2 antibody
anti-bcl-2 antibody Dilution Information image
Immunohistochemistry (IHC) of anti-bcl-2 antibody
anti-bcl-2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for bcl-2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,622 Da
NCBI Official Full Name
BCL-2
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Bcl-2
  [Similar Products]
NCBI Protein Information
apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene
UniProt Protein Name
Apoptosis regulator Bcl-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Bcl-2  [Similar Products]
UniProt Entry Name
BCL2_RAT
NCBI Summary for bcl-2
an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD]
UniProt Comments for bcl-2
Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Subunit structure: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Also interacts with APAF1, BBC3, BCL2L1, BNIPL, EI24, FKBP8, MRPL41, RAF-1, TP53BP2. Interacts with BAG1 in an ATP-dependent manner

Subcellular location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Tissue specificity: Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate.

Domain: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1

Post-translational modification: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A)

By similarity.Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity

By similarity.Monoubiquitinated by PARK2, leading to increase its stability

Sequence similarities: Belongs to the Bcl-2 family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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