• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-MET antibody :: Mouse anti-Human c-Met Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS245381
Unit / Price
  0.05 mL  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

c-Met, Monoclonal Antibody

Popular Item
Also Known As

Mouse Monoclonal (IgG2a) to Human c-Met

Product Synonym Names
Anti-c-Met Antibody (Cytoplasmic Domain) IHC-plus; AUTS9; C-Met; HGF/SF receptor; HGFR; Met kinase; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; HGF receptor; RCCP2; Tyrosine-protein kinase Met; Human c-Met
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1390
OMIM
gene 605074
3D Structure
ModBase 3D Structure for P08581
Clonality
Monoclonal
Isotype
IgG2a
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes c-Met.
Purity/Purification
Purified
Form/Format
PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Concentration
1.14 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide derived from cytoplasmic domain of human c-Met protein.
Immunogen Type
Synthetic peptide
Immunogen
c-Met antibody was raised against synthetic peptide derived from cytoplasmic domain of human c-Met protein.
Antigen Modification
Cytoplasmic Domain
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Other Notes
Small volumes of anti-MET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MET antibody
Met, an HGF Receptor/Met type protein kinase, is a cell-surface proto-oncogene that mediates the biological activities of hepatocyte growth factor. The protein is a disulfide-linked heterodimer comprised of two subunits: a 50 kD extracellular alpha subunit and a membrane-spanning 145 kD beta subunit that binds ligand. Met is an important regulator of cell proliferation and differentiation, organ regeneration, embryogenesis, and tumorogenesis.
Applications Tested/Suitable for anti-MET antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen)
Application Notes for anti-MET antibody
IHC-Fr (1:50), IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-MET antibody
Anti-c-Met antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-MET antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MET. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85,745 Da
NCBI Official Full Name
hepatocyte growth factor receptor isoform b
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met
  [Similar Products]
NCBI Protein Information
hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Synonym Protein Names
HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HGF receptor; SF receptor  [Similar Products]
UniProt Entry Name
MET_HUMAN
NCBI Summary for MET
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for MET
Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Oncoprotein; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; basal plasma membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; myoblast proliferation; protein amino acid autophosphorylation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; glucose homeostasis; liver development; signal transduction; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; endothelial cell morphogenesis; brain development; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000