• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CASP8 antibody :: Mouse anti-Human Caspase 8, Cleavage Site Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS601687
Unit / Price
Scan QR to view Datasheet
  0.1 mL  /  $595 +1 FREE 8GB USB
anti-CASP8 antibody
Product Name

Caspase 8, Cleavage Site (CASP8), Monoclonal Antibody

Full Product Name

Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5)

Product Synonym Names
Anti -Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (202098166..202152434). Location: 2q33-q34
OMIM
211980
3D Structure
ModBase 3D Structure for Q14790
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A52
Host
Mouse
Species Reactivity
Human
Specificity
Detects endogenous levels of the small subunit of caspase-8 resulting from cleavage at Asp384. Does not crossreact with full length caspase-8.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Immunogen
Synthetic peptide derived from the amino terminal sequence of p10 of human caspase-8 (KLH coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-CASP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CASP8 antibody
Apoptosis induced by CD95 (Fas/APO-1) and tumor necrosis factor receptor 1(TNFR1) activates caspase-8 (MACH/FLICE/Mch5), leading to the release of the active subunits p18 and p10 of caspase-8 (1-3). Activated caspase-8 is able to cleave and activate downstream caspases such as caspase-1, caspase-3, caspase-6 and caspase-7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
Applications Tested/Suitable for anti-CASP8 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-CASP8 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000
NCBI/Uniprot data below describe general gene information for CASP8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,391 Da[Similar Products]
NCBI Official Full Name
caspase 8
NCBI Official Synonym Full Names
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP8  [Similar Products]
NCBI Official Synonym Symbols
CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; FLJ17672; MGC78473
  [Similar Products]
NCBI Protein Information
caspase-8; FADD-like ICE; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; MACH-alpha-1/2/3 protein; apoptotic protease Mch-5; MACH-beta-1/2/3/4 protein; apoptotic cysteine protease; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FADD-homologous ICE/CED-3-like protease; caspase 8, apoptosis-related cysteine protease
UniProt Protein Name
Caspase-8
UniProt Synonym Protein Names
Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog
Protein Family
UniProt Gene Name
CASP8  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CASP8_HUMAN
NCBI Summary for CASP8
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]
UniProt Comments for CASP8
CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.61; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: nucleoplasm; mitochondrial outer membrane; neuron projection; cytoskeleton; mitochondrion; Noc1p-Noc2p complex; cytoplasm; CD95 death-inducing signaling complex; microtubule organizing center; cytosol; lipid raft

Molecular Function: peptidase activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; death receptor binding; protein complex binding; tumor necrosis factor receptor binding; cysteine-type peptidase activity

Biological Process: macrophage differentiation; viral reproduction; T cell activation; apoptosis; positive regulation of proteolysis; protein heterooligomerization; heart development; natural killer cell activation; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; proteolysis; response to estradiol stimulus; response to antibiotic; proteolysis involved in cellular protein catabolic process; angiogenesis; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; cell structure disassembly during apoptosis; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; MyD88-independent toll-like receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; response to ethanol; induction of apoptosis via death domain receptors; response to cobalt ion; toll-like receptor signaling pathway; innate immune response; response to cold; neural tube formation

Disease: Caspase 8 Deficiency; Lung Cancer; Breast Cancer; Hepatocellular Carcinoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000