• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CASP9 antibody :: Caspase-9 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS475166
Unit / Price
  0.1 mL  /  $335 +1 FREE 8GB USB
Product Name

Caspase-9 (CASP9), Monoclonal Antibody

Popular Item
Also Known As

Anti-Caspase-9 Mouse mAb

Product Synonym Names
APAF 3; APAF-3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP 9; CASP-9; CASP9; CASP9_HUMAN; Caspase 9 apoptosis related cysteine peptidase; Caspase 9 Dominant Negative; Caspase 9c; Caspase-9; Caspase-9 subunit p10; Caspase9; ICE LAP6; ICE like apoptotic protease 6; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9
Product Synonym Gene Name
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
416
OMIM
U56390 mRNA
3D Structure
ModBase 3D Structure for P55211
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D1-F2-E9
Host
Mouse
Source: Human
Species Reactivity
Human, Transfected
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide, and 50% glycerol.
Concentration
8mg/ml (lot specific)
Immunogen
Purified recombinant human Caspase-9 protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Caspase-9 and does not cross-react with related proteins.
Sequence Similarities
Belongs to the peptidase C14A family. Contains 1 CARD domain.
Subunit Structure
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Interacts with ABL1 (via SH3 domain); the interaction is direct and increases in the response of cells to genotoxic stress and ABL1/c-Abl activation. Interacts with NleF from pathogenic E Coli.
Other Notes
Small volumes of anti-CASP9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CASP9 antibody
Entrez Summary: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Applications Tested/Suitable for anti-CASP9 antibody
Western Blot (WB)
Application Notes for anti-CASP9 antibody
WB: 1:1000

Western Blot (WB) of anti-CASP9 antibody
Western blot detection of Caspase-9 in A549, MCF7, Jurkat, K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.
anti-CASP9 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CASP9 antibody
Western blot detection of Caspase-9 in CHO-K1 cell lysate (B) and CHO-K1 transfected by Caspase-9 (A) cell lysate using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49/37KDa.
anti-CASP9 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CASP9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49; 37
NCBI Official Full Name
caspase-9 isoform alpha
NCBI Official Synonym Full Names
caspase 9
NCBI Official Symbol
CASP9  [Similar Products]
NCBI Official Synonym Symbols
MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
  [Similar Products]
NCBI Protein Information
caspase-9
UniProt Protein Name
Caspase-9
UniProt Synonym Protein Names
Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3
Protein Family
UniProt Gene Name
CASP9  [Similar Products]
UniProt Synonym Gene Names
MCH6; CASP-9; APAF-3; ICE-LAP6  [Similar Products]
NCBI Summary for CASP9
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Comments for CASP9
CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms.

Protein type: Apoptosis; EC 3.4.22.62; Protease

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: apoptosome; cytosol

Molecular Function: cysteine-type endopeptidase activity; enzyme activator activity; peptidase activity; protein binding; protein kinase binding; SH3 domain binding

Biological Process: apoptosis; caspase activation via cytochrome c; DNA damage response, signal transduction; DNA damage response, signal transduction resulting in induction of apoptosis; platelet formation; response to DNA damage stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000