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Mouse anti-Human CD106 (VCAM-1) Monoclonal Antibody

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Catalog # MBS674161
Unit / Price
  100 Tests  /  $265 +1 FREE 8GB USB
anti-CD106 (VCAM-1) antibody
Product Name

CD106 (VCAM-1), Monoclonal Antibody

Also Known As

Mouse Anti-Human CD106/VCAM-1-PE

Product Synonym Names
Mouse Anti-Human CD106/VCAM-1 - Phycoerythrin-Labeled; Mouse Anti-Human CD106 (VCAM-1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
1.G11B1
Host
Mouse
Species Reactivity
Human
Specificity
Human CD106/VCAM-1
CD106, also known as INCAM-1, is a 100-110 kDa vascular adhesion cell adhesion molecule-1 (VCAM- 1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokineactivated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages and bone marrow stromal cells. Endothelial CD106 binds the integrins alpha 4 beta 1 (CD49d/CD29, VLA-4) and alpha 4 beta 7 and contributes to extravasation of lymphocytes, monocytes, basophils and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the beta 2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.1-4
Form/Format
R - phycoerythrin (R - PE) Conjugate
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of phosphate buffered saline containing 0.1% PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of anti-CD106 (VCAM-1) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD106 (VCAM-1) antibody
ELISA; Flow Cytometry; Immunohistochemistry (Frozen Sections); Western Blot
Application Notes for anti-CD106 (VCAM-1) antibody
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
Product References and Citations for anti-CD106 (VCAM-1) antibody
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD106 Section, Academic Press, New York, p. 386.
3. Thornhill, M.H., S.M. Wellicome, D.L. Mahiouz, J.S. Lanchbury, U. Kyan-Aung, and D.O. Haskard. 1991. J. Immunol. 146:592.
4. Bevilacqua, M.P. 1993. Annu. Rev. Immunol. 11:767.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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