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anti-CTLA4 antibody :: Mouse anti-Human CD152 Monoclonal Antibody

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Catalog # MBS8211324
Unit / Price
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  50 Assays  /  $215 +1 FREE 8GB USB
  100 Assays  /  $280 +1 FREE 8GB USB
Flow Cytometry (FC/FACS)
Product Name

CD152 (CTLA4), Monoclonal Antibody

Popular Item
Full Product Name

Anti-CD152 Antibody-FITC labled

Product Synonym Names
CD152; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD antigen CD152
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
109100
3D Structure
ModBase 3D Structure for P16410
Clonality
Monoclonal
Isotype
IgG2a
Host
Mouse
Species Reactivity
Human
Specificity
Recognises human CD152.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Conjugation
FITC
Directions for Use
1. Take 100 ul peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube.
2. Add 10 ul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light.
3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells.
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant.
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant.
6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
Preparation and Storage
Shipped and store at 4 degree C for one year. Do not freeze.
Other Notes
Small volumes of anti-CTLA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CTLA4 antibody
Mouse monoclonal antibody to CD152 (FITC)
Applications Tested/Suitable for anti-CTLA4 antibody
Immunofluoresence (IF), Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS) of anti-CTLA4 antibody
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD152 Antibody-FITC labled.
anti-CTLA4 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for CTLA4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,145 Da
NCBI Official Full Name
cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM
NCBI Official Synonym Full Names
cytotoxic T-lymphocyte-associated protein 4
NCBI Official Symbol
CTLA4  [Similar Products]
NCBI Official Synonym Symbols
CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
  [Similar Products]
NCBI Protein Information
cytotoxic T-lymphocyte protein 4
UniProt Protein Name
Cytotoxic T-lymphocyte protein 4
UniProt Synonym Protein Names
Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD_antigen: CD152
UniProt Gene Name
CTLA4  [Similar Products]
UniProt Synonym Gene Names
CD152; CTLA-4  [Similar Products]
UniProt Entry Name
CTLA4_HUMAN
NCBI Summary for CTLA4
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
UniProt Comments for CTLA4
CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; clathrin-coated endocytic vesicle; external side of plasma membrane

Molecular Function: protein binding

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; positive regulation of apoptosis; negative regulation of regulatory T cell differentiation; negative regulation of immune response; T cell costimulation; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus

Disease: Celiac Disease, Susceptibility To, 3; Autoimmune Lymphoproliferative Syndrome, Type V; Diabetes Mellitus, Insulin-dependent, 12; Hashimoto Thyroiditis; Systemic Lupus Erythematosus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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