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anti-CD19 antibody :: Mouse anti-Human CD19 Monoclonal Antibody

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Catalog # MBS674109
Unit / Price
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  100 Tests  /  $175 +1 FREE 8GB USB
anti-CD19 antibody
Product Name

CD19, Monoclonal Antibody

Full Product Name

Mouse Anti-Human CD19-FITC

Product Synonym Names
Mouse Anti-Human CD19 - FITC; Mouse Anti-Human CD19
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_000016.9 (28943260..28950668). Location: 16p11.2
OMIM
107265
3D Structure
ModBase 3D Structure for P15391
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
SJ25-C1
Host
Mouse
Species Reactivity
Human
Specificity
Human CD19
CD19 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is expressed at all stages of B cell differentiation except terminally differentiated plasma cells. It is also present on the cell surface of follicular dendritic cells. CD19 associates with CD21, CD81, Leu 13 and/or MHC Class II molecules to form a signal transduction complex on the B cell surface. This signaling complex modulates the activation threshold for the B cell antigen receptor (BCR).1-5
Form/Format
Fluorescein (FITC) Conjugate
Characterization
To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and Allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), Allophycocyanin -Cyanine 5.5 (APCCY 5.5) and Allophycocyanin -Cyanine 7 (APC-CY 7)conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of anti-CD19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD19 antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes for anti-CD19 antibody
Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
NCBI/Uniprot data below describe general gene information for CD19. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,128 Da[Similar Products]
NCBI Official Full Name
CD19
NCBI Official Synonym Full Names
CD19 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
B4; CVID3; MGC12802
  [Similar Products]
NCBI Protein Information
B-lymphocyte antigen CD19; OTTHUMP00000122551; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4
UniProt Protein Name
B-lymphocyte antigen CD19
UniProt Synonym Protein Names
B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
Protein Family
UniProt Gene Name
UniProt Entry Name
CD19_HUMAN
NCBI Summary for CD19
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]
UniProt Comments for CD19
Function: Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Subunit structure: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Ref.11

Subcellular location: Membrane; Single-pass type I membrane protein.

Post-translational modification: Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Ref.10 Ref.11 Ref.12

Involvement in disease: Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [

MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. Ref.13

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Sequence caution: The sequence AAA35533.1 differs from that shown. Reason: Frameshift at position 396.
Product References and Citations for anti-CD19 antibody
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.
2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD19 Section, Academic Press, New York, p. 179.
3. Tedder, T.F., L.J. Zhou, and P. Engel. 1994. Immunol. Today 14:437.
4. Fearon, D.T., and R.H. Carter. 1995. Annu. Rev. Immunol. 13:127.
5. Doody, G.M., P.W. Dempsey, and D.T. Fearson. 1996. Curr. Opin. Immunol. 8:378.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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