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anti-CD21 antibody :: Mouse anti-Human CD21 Monoclonal Antibody

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Catalog # MBS8505199
Unit / Price
  100 Tests  /  $310 +1 FREE 8GB USB
anti-CD21 antibody
Product Name

CD21, Monoclonal Antibody

Also Known As

Mouse anti-Human CD21, PE Conjugated mAb

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4ABU32
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Experimental Methods
1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Other Notes
Small volumes of anti-CD21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD21 antibody
4ABU32 reacts with CD21 protein, a 145 kDa receptor for C3d complement fragment and for Epstein-Barr virus (EBV), expressed on all mature B lymphocytes from sIg+ stage, then lost upon activation. It also present on follicular dendritic cells and some epithelia cells. It is reported CD21 weakly expressed on the subset of mature T-cells. CD21 plays a role in B cell activation and proliferation and is part of a large signal-transduction complex containing CD19, CD81and other molecules as regulator of complement activation.This clone is cross reactive with non-human primate.*CD21 is a phenotyping marker of lymphoma.
Applications Tested/Suitable for anti-CD21 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Product References and Citations for anti-CD21 antibody
1.Kishimoto, T. et al. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London. 2.Tsoukas CD and Lambris JD (1993). Immunology Today 14:56. 3.Cooper NR et al. (1988). Annu.Rev. Immunol. 6:85. 4.Ling NR et al. (1994) J. Immunol Methods 173:11.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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