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anti-CD22 antibody :: Mouse anti-Human CD22 Monoclonal Antibody

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Catalog # MBS249124
Unit / Price
  0.05 mL  /  $530 +1 FREE 8GB USB
Product Name

CD22, Monoclonal Antibody

Popular Item
Also Known As

Mouse Monoclonal [clone 1B1] (IgG1) to Human CD22

Product Synonym Names
Anti-CD22 Antibody (clone 1B1) IHC-plus; CD22; CD22 molecule; CD22 antigen; SIGLEC2; SIGLEC-2; T-cell surface antigen Leu-14; B-cell receptor CD22; BL-CAM; Human CD22
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
847
OMIM
107266
3D Structure
ModBase 3D Structure for P20273
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B1
Host
Mouse
Species Reactivity
Human
Specificity
Human CD22
Purity/Purification
Protein A/G Purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.
Antigen type
Recombinant Protein
Immunogen Type
Recombinant protein
Immunogen
CD22 antibody was raised against full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Other Notes
Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD22 antibody
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules .
Product Categories/Family for anti-CD22 antibody
Applications Tested/Suitable for anti-CD22 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-CD22 antibody
IF (1:100), IHC-P (5 ug/ml), WB (1:4000)

Immunohistochemistry (IHC) of anti-CD22 antibody
Anti-CD22 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD22 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-CD22 antibody
Anti-CD22 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD22.
anti-CD22 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-CD22 antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.
anti-CD22 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
B-cell receptor CD22 isoform 1
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
  [Similar Products]
NCBI Protein Information
B-cell receptor CD22; BL-CAM; CD22 antigen; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid binding Ig-like lectin 2; sialic acid-binding Ig-like lectin 2
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2  [Similar Products]
UniProt Entry Name
CD22_HUMAN
UniProt Comments for CD22
CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: cell adhesion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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