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anti-CD276 antibody :: Mouse anti-Human CD276 / B7-H3 Monoclonal Antibody

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Catalog # MBS245290
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

CD276 / B7-H3, Monoclonal Antibody

Popular Item
Also Known As

Mouse Monoclonal [clone MIH42] (IgG1) to Human CD276 / B7-H3

Product Synonym Names
Anti-CD276 / B7-H3 Antibody (clone MIH42) IHC-plus; CD276; 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276 antigen; CD276 molecule; Costimulatory molecule; B7H3; B7RP-2; Human CD276; B7-H3
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
316
OMIM
605715
3D Structure
ModBase 3D Structure for Q5ZPR3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MIH42
Host
Mouse
Species Reactivity
Human
Specificity
Detects CD276, a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
Purity/Purification
Affinity Purified
Form/Format
PBS, 0.09% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-CD276 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD276 antibody
B7-H3 is a member of the B7 family of proteins, which are, type I transmembrane glycoproteins containing two extracellular Ig domains. By engaging their cognate receptors on T cells, B7 family proteins provide accessory signals to T cells during T cell activation, which can be either stimulatory or inhibitory. B7-H3 is widely expressed and has been shown to be involved in bone formation via osteoblasts, allograft rejection and anti tumor response.
Applications Tested/Suitable for anti-CD276 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Flow Cytometry (FC/FACS)
Application Notes for anti-CD276 antibody
Flo (1:10 - 1:100), IHC-Fr (1:100), IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CD276 antibody
Anti-B7-H3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-CD276 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD276. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,165 Da
NCBI Official Full Name
CD276 antigen isoform b
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276  [Similar Products]
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
  [Similar Products]
NCBI Protein Information
CD276 antigen; B7 homolog 3; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Synonym Protein Names
4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD_antigen: CD276
Protein Family
UniProt Gene Name
CD276  [Similar Products]
UniProt Synonym Gene Names
B7H3; B7-H3  [Similar Products]
UniProt Entry Name
CD276_HUMAN
NCBI Summary for CD276
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for CD276
CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; positive regulation of T cell proliferation; immune response; positive regulation of bone mineralization; negative regulation of interferon-gamma biosynthetic process; positive regulation of interferon-gamma biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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