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anti-CD276 antibody :: anti-Human CD276 Monoclonal Antibody

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Catalog # MBS606057
Unit / Price
  0.2 mg  /  $645 +1 FREE 8GB USB
anti-CD276 antibody
Product Name

CD276, Monoclonal Antibody

Popular Item
Also Known As

Anti - CD276 (4Ig-B7-H3, B7 Homolog 3, B7-H3, B7H3, CD276 Antigen, CD276 Molecule, Costimulatory Molecule)

Product Synonym Names
CD276 (4Ig-B7-H3, B7 Homolog 3, B7-H3, B7H3, CD276 Antigen, CD276 Molecule, Costimulatory Molecule)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
534
Chromosome Location
Chromosome: 15; NC_000015.9 (73976622..74006859). Location: 15q23-q24
OMIM
605715
3D Structure
ModBase 3D Structure for Q5ZPR3
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10B1212
Host
Mouse
Source: Human
Species Reactivity
Human
Specificity
Recognizes human CD276.
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Concentration
1mg/ml (lot specific)
Immunogen
Human CD276.
Recommended Positive Control (IHC): Acetone fixed human tonsil.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-CD276 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD276 antibody
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma.

Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
Product Categories/Family for anti-CD276 antibody
Applications Tested/Suitable for anti-CD276 antibody
Immunohistochemistry, Flow Cytometry
Application Notes for anti-CD276 antibody
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Dilution: Flow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (frozen): 1:100
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for CD276. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,235 Da
NCBI Official Full Name
CD276 antigen isoform a
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276  [Similar Products]
NCBI Official Synonym Symbols
B7H3; B7-H3; 4Ig-B7-H3
  [Similar Products]
NCBI Protein Information
CD276 antigen; B7 homolog 3; OTTHUMP00000174913; OTTHUMP00000174914; costimulatory molecule
UniProt Protein Name
CD276 antigen
UniProt Synonym Protein Names
4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule
Protein Family
UniProt Gene Name
CD276  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CD276_HUMAN
NCBI Summary for CD276
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM]
UniProt Comments for CD276
CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; positive regulation of T cell proliferation; immune response; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma biosynthetic process
Product References and Citations for anti-CD276 antibody
• 1. Chapoval, A. I. et al. (2001) B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat. Immunol. 2: 269-274,
• 2. Sun, X. et al. (2003) Mouse B7-H3 induces antitumor immunity. Gene Ther. 10: 1728-1734,
• 3. Ma, L. et al. (2007) Complete eradication of hepatocellular carcinomas by combined vasostatin gene therapy and B7H3-mediated immunotherapy. J. Hepatol. 46: 98-106.As reported

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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