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anti-CD44 antibody :: Mouse anti-Human CD44 Monoclonal Antibody

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Catalog # MBS245495
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

CD44, Monoclonal Antibody

Also Known As

Mouse Monoclonal [clone MEM-85] (IgG2b) to Human CD44

Product Synonym Names
Anti-CD44 Antibody (clone MEM-85) IHC-plus; CD44; CDW44; CD44 antigen; Cell surface glycoprotein CD44; CSPG8; ECMR-III; Epican; HUTCH-I; LHR; MDU2; MC56; MIC4; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; PGP-I; Pgp1; Phagocytic glycoprotein I; MDU3; CD44R; HCELL; Human CD44
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
742
OMIM
gene 609027
3D Structure
ModBase 3D Structure for P16070
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
MEM-85
Host
Mouse
Species Reactivity
Human
Specificity
Reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kD transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Purity/Purification
Protein A Purified
Form/Format
PBS, 15 mM sodium azide, approx., pH 7.4.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Leukocytes of a patient suffering from LGL Type Leukaemia.
Immunogen
CD44 antibody was raised against leukocytes of a patient suffering from LGL Type Leukaemia.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Other Notes
Small volumes of anti-CD44 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD44 antibody
CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis.
Applications Tested/Suitable for anti-CD44 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CD44 antibody
ELISA, Flo, IHC-P (5 ug/ml), IP, WB (2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-CD44 antibody
Anti-CD44 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-CD44 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD44. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,635 Da
NCBI Official Full Name
CD44 antigen isoform 1
NCBI Official Synonym Full Names
CD44 molecule (Indian blood group)
NCBI Official Symbol
NCBI Official Synonym Symbols
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
  [Similar Products]
NCBI Protein Information
CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system
UniProt Protein Name
CD44 antigen
UniProt Synonym Protein Names
CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD_antigen: CD44
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I  [Similar Products]
UniProt Entry Name
CD44_HUMAN
NCBI Summary for CD44
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
UniProt Comments for CD44
CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: Golgi apparatus; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; hyaluronic acid binding; hyalurononglucosaminidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; Wnt receptor signaling pathway; glycosaminoglycan metabolic process; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; pathogenesis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; extracellular matrix disassembly; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion; ureteric bud branching; cartilage development; carbohydrate metabolic process; blood coagulation; leukocyte migration; hyaluronan metabolic process; healing during inflammatory response; negative regulation of apoptosis

Disease: Blood Group, Indian System
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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