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anti-Cd44 antibody :: Mouse anti-Human CD44V7 Monoclonal Antibody

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Catalog # MBS602260
Unit / Price
  0.1 mg  /  $580 +1 FREE 8GB USB
anti-Cd44 antibody
Product Name

CD44V7, Monoclonal Antibody

Popular Item
Also Known As

CD44V7 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

Product Synonym Names
Anti -CD44V7 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000068.7 (102811141..102901665, complement). Location: 2 E2; 2 54.13 cM
3D Structure
ModBase 3D Structure for P15379
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H1353
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes an epitope encoded by exon v7 on the variant portion of human CD44.
Purity/Purification
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.01% thimerosal.
Immunogen
Glutathione S Transferase (GST) fusion protein corresponding to the variable domains (v3 to v10) of human CD44
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-Cd44 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Cd44 antibody
Recent studies indicate that the expression of CD44 on the cell surface changes profoundly during tumor metastasis, particularly during the progression of various carcinomas. Sequencing analysis reveals that CD44 exists in different forms (>15 isoforms have been identified) that are variants of the common or standard hemopoietic form CD44s. Specifically, the human CD44 gene contains 19 exons, out of which 10 exons can potentially be alternatively spliced. In particular, CD44s contains exons 1-15 and exons 15-19. The other exons (e.g. 6-14) are also spliced in a variety of different cells. For example, epithelial cells contain exons 12-14 inserted into the CD44s transcripts. This isoform has been designated as CD44E. Other CD44 variants CD44v) have the same sequences at the two ends of the molecule, but differ in a middle region (with different numbers of additional exons) located on the external side of the cell membrane. It has been suggested that expression of CD44 variants with the additional exon 11 (so-called v7) is responsible for tumor progression and metastasis. In fact, CD44v7 expression has been used as an indicator of metastasis.
Product Categories/Family for anti-Cd44 antibody
Applications Tested/Suitable for anti-Cd44 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Cd44 antibody
Suitable for us ein Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (frozen): 1:50
Immunohistochemistry (paraffin): 1:50 Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
Flow Cytometry: 1:50. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal working dilutions to be determined by researcher.
Immunohistochemistry Positive Control:
Skin
NCBI/Uniprot data below describe general gene information for Cd44. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85,617 Da[Similar Products]
NCBI Official Full Name
CD44 antigen
NCBI Official Synonym Full Names
CD44 antigen
NCBI Official Symbol
NCBI Official Synonym Symbols
Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
  [Similar Products]
NCBI Protein Information
CD44 antigen; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; lymphocyte antigen 24; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor
UniProt Protein Name
CD44 antigen
UniProt Synonym Protein Names
Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Lymphocyte antigen 24; Ly-24; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ly-24; ECMR-III; Ly-24; PGP-1; PGP-I  [Similar Products]
UniProt Entry Name
CD44_MOUSE
UniProt Comments for Cd44
CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Cellular Component: Golgi apparatus; protein complex; focal adhesion; cell surface; membrane; basolateral plasma membrane; apical plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus; external side of plasma membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; cytokine binding; hyaluronic acid binding; phosphoprotein binding; protein kinase binding; hyalurononglucosaminidase activity; epidermal growth factor receptor binding

Biological Process: cell migration; Wnt receptor signaling pathway; positive regulation of heterotypic cell-cell adhesion; positive regulation of neutrophil apoptosis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; ureteric bud branching; regulation of cell growth; cell adhesion; healing during inflammatory response; positive regulation of adaptive immune response; neurite development; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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