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anti-CD53 antibody :: Mouse CD53 Monoclonal Antibody

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Catalog # MBS439289
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
anti-CD53 antibody
Product Name

CD53, Monoclonal Antibody

Popular Item
Full Product Name

CD53 (TSPAN25) Mouse Monoclonal Antibody

Product Synonym Names
CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
151525
3D Structure
ModBase 3D Structure for P19397
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
63-5A3
Host
Mouse
Species Reactivity
Human.
Others not tested
Specificity
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cell Surface
Immunogen
Human Sezary cells
Hu-Chromosome Location
1p13
Positive Control
Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-CD53 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD53 antibody
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD53 antibody
Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
NCBI/Uniprot data below describe general gene information for CD53. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33-55kDa
NCBI Official Full Name
leukocyte surface antigen CD53 isoform 1
NCBI Official Synonym Full Names
CD53 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
MOX44; TSPAN25
  [Similar Products]
NCBI Protein Information
leukocyte surface antigen CD53
UniProt Protein Name
Leukocyte surface antigen CD53
UniProt Synonym Protein Names
Cell surface glycoprotein CD53; Tetraspanin-25; Tspan-25; CD_antigen: CD53
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MOX44; TSPAN25; Tspan-25  [Similar Products]
UniProt Entry Name
CD53_HUMAN
NCBI Summary for CD53
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Comments for CD53
CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: immunological synapse; integral to plasma membrane; intercellular junction; plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction
Product References and Citations for anti-CD53 antibody
Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989 Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997. Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716. Mannion BA et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin 4 beta 1 (CD49d/CD29). J Immunol 1996, 157(5):2039-2047. Carmo AM et al. Association of the transmembrane 4-superfamily molecule CD53 with a tyrosine phosphatase activity. Eur J Immunol 1995, 25(7):2090-2095. Mollinedo F et al. Recurrent infectious diseases in human CD53 deficiency. Clin Diagn Lab Immunol 1997, 4(2):229-231

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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