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anti-NCAM1 antibody :: Mouse anti-Human CD56 / NCAM1 / NKH1 Monoclonal Antibody

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Catalog # MBS439343
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

CD56 / NCAM1 / NKH1, Monoclonal Antibody

Popular Item
Full Product Name

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Mouse Monoclonal Antibody

Product Synonym Names
NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
116930
3D Structure
ModBase 3D Structure for P13591
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
NCAM1/784
Host
Mouse
Species Reactivity
Human
Specificity
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cell Surface
Immunogen
Recombinant human NCAM1 protein
Hu-Chromosome Location
11q23.1
Positive Control
Cerebellum, Pancreas, Neuroblastoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-NCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NCAM1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NCAM1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-NCAM1 antibody
Formalin-fixed, paraffin-embedded human Colon stained with CD56 Monoclonal Antibody (NCAM1/784)
anti-NCAM1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for NCAM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
180; 145; 125kDa
NCBI Official Full Name
neural cell adhesion molecule 1 isoform 1
NCBI Official Synonym Full Names
neural cell adhesion molecule 1
NCBI Official Symbol
NCAM1  [Similar Products]
NCBI Official Synonym Symbols
CD56; NCAM; MSK39
  [Similar Products]
NCBI Protein Information
neural cell adhesion molecule 1
UniProt Protein Name
Neural cell adhesion molecule 1
UniProt Synonym Protein Names
CD_antigen: CD56
UniProt Gene Name
NCAM1  [Similar Products]
UniProt Synonym Gene Names
NCAM; N-CAM-1; NCAM-1  [Similar Products]
UniProt Entry Name
NCAM1_HUMAN
NCBI Summary for NCAM1
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
UniProt Comments for NCAM1
NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; Golgi membrane; membrane; plasma membrane

Molecular Function: Ras guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; MAPKKK cascade
Product References and Citations for anti-NCAM1 antibody
Endo, C., et al. 2009. Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung. Acta Cytol. 53: 36-40

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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