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anti-CD62L antibody :: Mouse anti-Human CD62L Monoclonal Antibody

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Catalog # MBS666746
Unit / Price
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  120 Tests  /  $310 +1 FREE 8GB USB
Binding
Product Name

CD62L, Monoclonal Antibody

Popular Item
Full Product Name

anti-human CD62L-FITC

Product Synonym Names
Monoclonal anti-human CD62L (L-selectin)/FITC
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG2ak
Clone Number
LAM1-116
Host
Mouse
Species Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Performance
Five x 10A5 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD62L/FITC at a 1:50 dilution factor (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD62L antibody.
Conjugation
FITC
Production
Protein A purified antibody from tissue culture supernatant was reacted with FITC. Unconjugated FITC was separated from antibody/FITC conjugate by desalting column. The antibody/FITC conjugate is at 0.5 mg/ml with a Fluorescein: antibody molar ratio of 13.8.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
Mouse pre-B cells expressing human L-selectin
Performance
Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-D62L/FITC t a 1:50 dilution factor (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse gG2a/FITC negative control (Catalog #MBS666101) at a similar concentration. Binding was blocked hen cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD62L antibody .
Preparation and Storage
Store at 2 - 5°C. Freeze/Thawing is not recommended. Protect from light.
Other Notes
Small volumes of anti-CD62L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD62L antibody
Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2). Antibody LAM1-116 recognizes the lectin binding domain of CD62L (2). Antibody LAM1-116 blocks CD62L function and induces expression of beta-1 and beta-2 integrins (1).
Applications Tested/Suitable for anti-CD62L antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD62L antibody
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)

anti-CD62L antibody Binding image
Product References and Citations for anti-CD62L antibody
(1). T.A. Springer, (1995) Annu Rev Physiol 57: 827-872. (2). D.A. Steeber, et al, (1997) J Immunol 159: 952-963. (3). Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 420-424.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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