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anti-CD8 antibody :: Rabbit anti-Human CD8 Monoclonal Antibody

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Catalog # MBS684124
Unit / Price
  1 ml (Prediluted)  /  $175 +1 FREE 8GB USB
  0.04 mL  /  $185 +1 FREE 8GB USB
  0.1 mL  /  $360 +1 FREE 8GB USB
  7 ml (Prediluted)  /  $390 +1 FREE 8GB USB
  0.2 mL  /  $490 +1 FREE 8GB USB
  15 ml (Prediluted)  /  $570 +1 FREE 8GB USB
  0.5 mL  /  $615 +1 FREE 8GB USB
  1 mL  /  $955 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

CD8, Monoclonal Antibody

Popular Item
Also Known As

Anti - CD8

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
235
Chromosome Location
Chromosome: 2; NC_000002.12 (86784605..86808396, complement). Location: 2p12
OMIM
186910
3D Structure
ModBase 3D Structure for P01732
Clonality
Monoclonal
Clone Number
P17-V
Host
Rabbit
Species Reactivity
Human
Specificity
Human
Immunogen
Peptide derived from C-terminal region of human CD8
Storage Buffer
20 mM Tris-HCI, pH 8.0
Stabilizer
20 mg/ml BSA
Preservative
0.05% NaN3
Immunogen
Peptide derived from C-terminal region of human CD8. Antibody recognizes the epitope between Pro218 - Tyr234.
Positive control
human tonsil tissue
Cellular localization
membrane
IHC-P PROTOCOL–INSTRUCTION MANUAL
1.Deparaffinize the section in 3 changes of xylene, 5 minutes each.
2.Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3.Rinse in distilled water.
4.Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5.Wash in distilled water.
6.For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0,0.05% Tween-20*, and incubatein water bath at 96-98ºC for 20-25 minutes.
7.Transfer the slide to room temperature and let it cool down for 15 minutes in the buffer used in the step #6
8.Rinse in distilled water.
9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.READY TO USE (RTU): Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.
11.Wash twice 5 minutes with buffer A.
12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard mmunohistochemistry protocol .
13.Wash twice 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 -3 minutes.
15.Wash in water –10 minutes.
16.Stain in hematoxylin for 5 minutes.
17.Wash in water –10 minutes.8.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19.Wash the section in 2 changes of xylene for 2 minutes each.
20.Mount the slide for observation.
PRECAUTIONS
1.We strongly recommend to use DB Primary Antibody Diluent eventually alternative diluent (containing protease free BSA at the concentrations ? 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be oided.
2.Centrifuge the vial before use.3.Intended for professional In Vitro Diagnostic use in laboratories.
4.Do not use after expiration date stamped on vial label.
5.Avoid contamination of the reagent.
6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8.Disposal of waste material must be conducted in accordance with local regulations.
9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Store at +4°C. Do not freeze!
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of anti-CD8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD8 antibody
Immunohistochemistry (IHC) - Formalin/Paraffin

Immunohistochemistry (IHC) of anti-CD8 antibody
CD8 expression in T-lymphocytes of the palatine tonsil. Formalin fixed, paraffin embedded human tissue (4 ?msection) stained with anti -CD8 (MBS684124) monospecific clonal antibody
anti-CD8 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
T-cell surface glycoprotein CD8 alpha chain
NCBI Official Synonym Full Names
CD8a molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
CD8; MAL; p32; Leu2
  [Similar Products]
NCBI Protein Information
T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
UniProt Protein Name
T-cell surface glycoprotein CD8 alpha chain
UniProt Synonym Protein Names
T-lymphocyte differentiation antigen T8/Leu-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
CD8A_HUMAN
NCBI Summary for CD8
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Comments for CD8
Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in disease: CD8 deficiency, familial (CD8 deficiency) [MIM:608957]: An immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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