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anti-CD81 antibody :: Mouse anti-Human CD81 Monoclonal Antibody

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Catalog # MBS9217050
Unit / Price
  0.05 mL  /  $150 +1 FREE 8GB USB
  0.2 mL  /  $340 +1 FREE 8GB USB
Product Name

CD81, Monoclonal Antibody

Popular Item
Also Known As

CD81 Antibody

Product Synonym Names
CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD81; CD81; TAPA1; TSPAN28
Product Synonym Gene Name
TAPA1; TSPAN28[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
165
OMIM
186845
3D Structure
ModBase 3D Structure for P60033
Clonality
Monoclonal
Isotype
IgG1,k
Host
Mouse
Species Reactivity
Human
Specificity
This CD81 antibody is generated from a mouse immunized with recombinant protein from the human region of human CD81.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Cellular Location
Membrane; Multi-pass membrane protein.
Clone Names
1668CT270.93.9
Tissue Location
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-CD81 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD81 antibody
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.
Applications Tested/Suitable for anti-CD81 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD81 antibody
WB:~~1:4000

Test Data of anti-CD81 antibody
All lanes:
Anti-CD81 Antibody at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Ramos whole cell lysate
Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ?g per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-CD81 antibody Test Data image
Western Blot (WB) of anti-CD81 antibody
Anti-CD81 Antibody at 1:4000 dilution + human testis whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-CD81 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-CD81 antibody
Anti-CD81 Antibody at 1:4000 dilution + rat cerebellum whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 26 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-CD81 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CD81. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25809
NCBI Official Full Name
CD81 antigen isoform 2
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
  [Similar Products]
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
UniProt Synonym Protein Names
26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28; Tspan-28; CD_antigen: CD81
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28  [Similar Products]
UniProt Entry Name
CD81_HUMAN
NCBI Summary for CD81
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Comments for CD81
CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: apical plasma membrane; focal adhesion; immunological synapse; integral to plasma membrane; membrane; plasma membrane; vesicle

Molecular Function: protein binding

Biological Process: activation of MAPK activity; cell proliferation; cell surface receptor linked signal transduction; entry of virus into host cell; positive regulation of 1-phosphatidylinositol 4-kinase activity; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein localization; receptor internalization; regulation of growth; regulation of immune response; regulation of protein stability; response to wounding; virion attachment, binding of host cell surface receptor

Disease: Immunodeficiency, Common Variable, 6
Product References and Citations for anti-CD81 antibody
Oren R.,et al.Mol. Cell. Biol. 10:4007-4015 (1990). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Livingston R.J.,et al.Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases. Taylor T.D.,et al.Nature 440:497-500 (2006). Takahashi S.,et al.J. Immunol. 145:2207-2213 (1990).

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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