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anti-CDC5L antibody :: Mouse anti-Human CDC5L Monoclonal Antibody

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Catalog # MBS120141
Unit / Price
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  0.1 mg  /  $345 +1 FREE 8GB USB
Product Name

CDC5L, Monoclonal Antibody

Full Product Name

Mouse monoclonal antibody Anti-Human CDC5L

Product Synonym Names
Homo sapiens CDC5 cell division cycle 5-like (S. pombe) (CDC5L); CEF1; hCDC5; PCDC5RP; KIAA0432; dJ319D22.1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602868
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2136C1a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-CDC5L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CDC5L antibody
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-CDC5L antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-CDC5L antibody
Western blot analysis of immunized recombinant protein, using anti-CDC5L monoclonal antibody.
anti-CDC5L antibody Quality Control image
Quality Control #2 of anti-CDC5L antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-CDC5L antibody Quality Control #2 image
Western Blot (WB) of anti-CDC5L antibody
Detection of CDC5L by Western blot.

Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa
anti-CDC5L antibody Western Blot (WB) (WB) image
Immunoprecipitation (IP) of anti-CDC5L antibody
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CDC5L mAb. [Lot No. 2136C1a-1]

Predicted molecular weight: 92 kDa
anti-CDC5L antibody Immunoprecipitation (IP) (IP) image
Flow Cytometry (FC/FACS) of anti-CDC5L antibody
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2136C1a-1]
anti-CDC5L antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunocytochemistry (ICC) of anti-CDC5L antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDC5L mAb. [Lot No. 2136C1a-1]
anti-CDC5L antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for CDC5L. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,251 Da
NCBI Official Full Name
Homo sapiens cell division cycle 5-like (CDC5L), mRNA
NCBI Official Synonym Full Names
cell division cycle 5-like
NCBI Official Symbol
CDC5L  [Similar Products]
NCBI Official Synonym Symbols
CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
  [Similar Products]
NCBI Protein Information
cell division cycle 5-like protein; CDC5 cell division cycle 5-like; Cdc5-related protein; dJ319D22.1 (CDC5-like protein); pombe cdc5-related protein
UniProt Protein Name
Cell division cycle 5-like protein
UniProt Synonym Protein Names
Pombe cdc5-related protein
UniProt Gene Name
CDC5L  [Similar Products]
UniProt Entry Name
CDC5L_HUMAN
NCBI Summary for CDC5L
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]
UniProt Comments for CDC5L
CDC5L: is a spliceosome protein that binds DNA and that also regulates transcription. Is a positive regulator of cell cycle G2/M progression. Is an essential component of non-snRNA spliceosomes and is required for the second catalytic step of pre-mRNA splicing. Binds adeno-pre-mRNA in an ATP-stimulated manner. Is part of a spliceosomal 'core' complex that includes CDC5L, SF2, PRLI, SPF27, HSP7C, PRPF19/SNEV, GCN1L. Identified in the spliceosome C complex. Has been associated with aneuploidy in aging cells and in tumorigenesis. The genomic region containing the gene for CDC5L (6p21.1) is frequently amplified in osteosarcomas.

Protein type: Spliceosome; RNA processing; DNA-binding; RNA splicing; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; gene expression; DNA repair
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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