• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-CEACAM8 antibody :: Mouse anti-Human CEACAM8 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS468020
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $505 +1 FREE 8GB USB
Product Name

CEACAM8, Monoclonal Antibody

Full Product Name

anti-human CEACAM8 monoclonal antibody

Product Synonym Names
CD66b; NCA-95
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
114890
3D Structure
ModBase 3D Structure for P31997
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GM2H6
Host
Mouse
Species Reactivity
Human
Specificity
anti-human CEACAM8 (CD66b/NCA-95)
Purity/Purification
Protein G
Form/Format
Purified
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human CEACAM8
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Other Notes
Small volumes of anti-CEACAM8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CEACAM8 antibody
Background: CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B domains.
Applications Tested/Suitable for anti-CEACAM8 antibody
Flow Cytometry (FC/FACS), CELISA, ELISA (EIA)
Application Notes for anti-CEACAM8 antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Testing Data of anti-CEACAM8 antibody
Fig.1: FACS analysis of BOSC23 cells using GM2H6 Cat.# GM- 0512. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.
anti-CEACAM8 antibody Testing Data image
Testing Data #2 of anti-CEACAM8 antibody
Fig. 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8: Tet2; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).
anti-CEACAM8 antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for CEACAM8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,154 Da[Similar Products]
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 8
NCBI Official Symbol
CEACAM8  [Similar Products]
NCBI Official Synonym Symbols
CD67; CGM6; CD66b; NCA-95
  [Similar Products]
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 8; CD67 antigen; carcinoembryonic antigen CGM6; non-specific cross-reacting antigen NCA-95; carcinoembryonic antigen gene family member 6
UniProt Synonym Protein Names
CD67 antigen; Carcinoembryonic antigen CGM6; Non-specific cross-reacting antigen NCA-95; CD_antigen: CD66b
UniProt Gene Name
CEACAM8  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
CEAM8_HUMAN
UniProt Comments for CEACAM8
CEACAM8: Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Biological Process: immune response; blood coagulation; leukocyte migration
Product References and Citations for anti-CEACAM8 antibody
1. Zimmermann W (2002). Carcinoembryonic antigen. In Wiley Encyclopedia of Molecular Medicine (T. Creighton, ed.), John Wiley & Sons Inc., New York, USA, pp. 459-462.
2. Zhao L, Xu S, Fjaertoft G, Pauksen K, Hakansson L and Venge P (2004). An enzyme-linked immunosorbent assay for human carcinoembryonic antigen-related cell adhesion molecule 8, a biological marker of granulocyte activities in vivo. J. Immunol. Methods 293(1-2):207-14
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin. Cancer Biol. 9, 67-81.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000