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anti-CHI3L1 antibody :: Mouse anti-Human CHI3L1 Monoclonal Antibody

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Catalog # MBS200140
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

CHI3L1, Monoclonal Antibody

Popular Item
Also Known As

CHI3L1 antibody

Product Synonym Names
chitinase 3-like 1 precursor; chitinase 3-like 1 (cartilage glycoprotein-39); GP39; YKL40
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
383
OMIM
M80927 mRNA
3D Structure
ModBase 3D Structure for P36222
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2C10
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human CHI3L1 (22-383aa) purified from E. coli
Clone Note
Anti-human CHI3L1 mAb, clone AT2C10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CHI3L1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-CHI3L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CHI3L1 antibody
Chitinase 3-Like-1 (CHI3L1) is a recently discovered human glycoprotein which is related in amino acid sequence to the chitinase protein family, but has no chitinase activity. CHI3L1 is upregulated in a number of cancers and in non-neoplatic disease states characterized by chronic inflammation and tissue remodeling. Its pattern of expression is associated with pathogenic processes related to inflammation, extracellular tissue remodeling, fibrosis and solid carcinomas. CHI3L1 may play an important role in the capacity of cells to respond to and cope with changes in their environment.
Product Categories/Family for anti-CHI3L1 antibody
Applications Tested/Suitable for anti-CHI3L1 antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes for anti-CHI3L1 antibody
WB: 1:250 ~ 1500.
Recommended starting dilution is 1:250

Western Blot (WB) of anti-CHI3L1 antibody
Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: SW480 cell lysate
anti-CHI3L1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for CHI3L1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,625 Da
NCBI Official Full Name
chitinase-3-like protein 1
NCBI Official Synonym Full Names
chitinase 3 like 1
NCBI Official Symbol
CHI3L1  [Similar Products]
NCBI Official Synonym Symbols
GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
  [Similar Products]
NCBI Protein Information
chitinase-3-like protein 1
UniProt Protein Name
Chitinase-3-like protein 1
UniProt Synonym Protein Names
39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; YKL-40
Protein Family
UniProt Gene Name
CHI3L1  [Similar Products]
UniProt Synonym Gene Names
CGP-39; GP-39; hCGP-39  [Similar Products]
NCBI Summary for CHI3L1
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
UniProt Comments for CHI3L1
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Product References and Citations for anti-CHI3L1 antibody
Kazakova MH, et al., (2009) Folia Med. 51 (1):5-14; Bonneh-Barkay D, et al., (2008) Am J Pathol. 173 (1):130-43

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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