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anti-CX3CR1 antibody :: Mouse anti-Human CX3CR1 Monoclonal Antibody

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Catalog # MBS632828
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  100 Tests  /  $615 +1 FREE 8GB USB
anti-CX3CR1 antibody
Product Name

CX3CR1, Monoclonal Antibody

Popular Item
Full Product Name

CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (Carboxyfluorescein)

Product Synonym Names
Anti -CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokine Receptor 1, C-X3-C CKR-1, CX3C CKR-1, CMK-BRL-1, CMKBLR1, CMKDR1, Fractalkine Receptor, GPR13, GPRV28, V28) (Carboxyfluorescein)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 3; NC_000003.11 (39304985..39323226, complement). Location: 3p21; 3p21.3
OMIM
601470
3D Structure
ModBase 3D Structure for P49238
Clonality
Monoclonal
Isotype
IgG
Clone Number
9H106
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human CX3CR1.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein.
Immunogen
Recombinant protein corresponding to the extracellular domain of human CX3CR1 expressed in E. coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.
Other Notes
Small volumes of anti-CX3CR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CX3CR1 antibody
CX3CR1 is a 40kD seven transmembrane chemokine receptor that is expressed on several T cell subsets, monocytes, macrophages, microglia and epithelial cells. CX3CR1 binding to membrane bound or soluble CX3CR1/Fractalkine promotes inflammatory responses by inducing monocyte adhesion to endothelial cells and macrophage activation. CX3CR1 polymorphisms are associated with the development of chronic inflammatory disorders. Alternately spliced isoforms have extended N-terminal extracellular regions that increase the potency of CX3CR1 as a fusion co-receptor for HIV-1. Human CX3CR1 shares 82% amino acid sequence identity with mouse and rat CX3CR1.
Applications Tested/Suitable for anti-CX3CR1 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CX3CR1 antibody
Suitable for Flow Cytometry. Other Appliucacations not tested.
Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Tested using human peripherial blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C8299-01E to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for flow cytometry analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled mouse IgG1 antibody.
NCBI/Uniprot data below describe general gene information for CX3CR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40,396 Da[Similar Products]
NCBI Official Full Name
CX3C chemokine receptor 1
NCBI Official Synonym Full Names
chemokine (C-X3-C motif) receptor 1
NCBI Official Symbol
CX3CR1  [Similar Products]
NCBI Official Synonym Symbols
V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
  [Similar Products]
NCBI Protein Information
CX3C chemokine receptor 1; CMK-BRL1; CMK-BRL-1; C-X3-C CKR-1; OTTHUMP00000209587; OTTHUMP00000209588; OTTHUMP00000209589; fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; chemokine (C-X3-C) receptor 1; beta chemokine receptor-like 1; chemokine (C-C) receptor-like 1
UniProt Protein Name
CX3C chemokine receptor 1
UniProt Synonym Protein Names
Beta chemokine receptor-like 1; CMK-BRL-1; CMK-BRL1; Fractalkine receptor; G-protein coupled receptor 13; V28
Protein Family
UniProt Gene Name
CX3CR1  [Similar Products]
UniProt Synonym Gene Names
CMKBRL1; GPR13  [Similar Products]
UniProt Entry Name
CX3C1_HUMAN
NCBI Summary for CX3CR1
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for CX3CR1
CX3CR1: Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. Defects in CX3CR1 are a cause of susceptibility to age- related macular degeneration type 12 (ARMD12). ARMD12 is a form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Cell adhesion; Membrane protein, integral; Receptor, cytokine; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: neuron projection; integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: protein binding; chemokine receptor activity; C-X3-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of angiogenesis; cerebral cortex cell migration; viral reproduction; response to wounding; cellular defense response; chemotaxis; cell adhesion; negative regulation of cell migration; macrophage chemotaxis; microglial cell activation during immune response

Disease: Macular Degeneration, Age-related, 12; Coronary Heart Disease, Susceptibility To, 1; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-CX3CR1 antibody
 Reference Product  PubMed Publications
 CX3CL1 antibody  >281 publications with CX3CR1 and CX3CL1
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