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anti-CFTR antibody :: Mouse anti-Human Cystic Fibrosis Transmembrane Conductance Regulator Monoclonal Antibody

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Catalog # MBS604644
Unit / Price
  0.2 mg  /  $765 +1 FREE 8GB USB
anti-CFTR antibody
Product Name

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), Monoclonal Antibody

Also Known As

Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Product Synonym Names
Anti -Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 7; NC_000007.13 (117120017..117308719). Location: 7q31.2
OMIM
219700
3D Structure
ModBase 3D Structure for P13569
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4i35
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes CFTR, Mr 170kDa. Species Crossreactivity: Human.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in ~530ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Immunogen
Fusion Protein corresponding to residues 1197-1480 of human cystic ?brosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-CFTR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CFTR antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-CFTR antibody
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot.
Dilution: Immunoprecipitation: Reported to immunoprecipitate CFTR.1
Immunohistochemistry: Reported to immunostain CFTR in human pancreatic tissue sections.1
Immunoblot Analysis: 0.5-2ug/ml detects CFTR from 20-50ug of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2ug/ml of a previous lot detected CFTR from CFTR-transfected BHK2. Note: Do not boil the lysate. Instead incubate at 37 degree C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE. T84 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CFTR (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
NCBI/Uniprot data below describe general gene information for CFTR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
168,142 Da[Similar Products]
NCBI Official Full Name
cystic fibrosis transmembrane conductance regulator
NCBI Official Synonym Full Names
cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
NCBI Official Symbol
NCBI Official Synonym Symbols
CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
  [Similar Products]
NCBI Protein Information
cystic fibrosis transmembrane conductance regulator; OTTHUMP00000024694; OTTHUMP00000196524; cAMP-dependent chloride channel; channel conductance-controlling ATPase; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7
UniProt Protein Name
Cystic fibrosis transmembrane conductance regulator
UniProt Synonym Protein Names
ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49); cAMP-dependent chloride channel
UniProt Gene Name
UniProt Synonym Gene Names
ABCC7; CFTR  [Similar Products]
UniProt Entry Name
CFTR_HUMAN
NCBI Summary for CFTR
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq]
UniProt Comments for CFTR
CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Transporter; Membrane protein, multi-pass; Channel, chloride; Transporter, ABC family; Hydrolase; EC 3.6.3.49; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: recycling endosome; protein complex; cytoplasmic vesicle membrane; cell surface; microvillus; early endosome membrane; basolateral plasma membrane; cytoplasm; early endosome; apical plasma membrane; plasma membrane

Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; chloride channel inhibitor activity; enzyme binding; chloride transmembrane transporter activity; ATP-binding and phosphorylation-dependent chloride channel activity; channel-conductance-controlling ATPase activity; ATP binding; PDZ domain binding

Biological Process: response to drug; intracellular pH elevation; response to peptide hormone stimulus; cholesterol transport; iodide transport; transepithelial chloride transport; water transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; cellular response to hormone stimulus; transport; response to estrogen stimulus; bicarbonate transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development

Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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