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anti-DDX5 antibody :: Mouse anti-Human DDX5 Monoclonal Antibody

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Catalog # MBS120235
Unit / Price
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  0.1 mg  /  $395 +1 FREE 8GB USB
Product Name

DDX5, Monoclonal Antibody

Popular Item
Full Product Name

Mouse monoclonal antibody Anti-Human DDX5

Product Synonym Names
Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5); p68; HLR1; G17P1; HUMP68; DKFZp686J01190
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
180630
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2257C3a
Host
Mouse
Species Reactivity
Human
Form/Format
Mouse monoclonal anti-human DDX5antibody in PBS (3.0 mM KCl, 1.5 mM KH PO , 140 mM NaCl, 8.0 mM Na HPO (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3)
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Immunogen
Recombinant
Sterility
Filtered through a 0.22 um membrane.
Disposal
This antibody solution contains sodium azide (NaN )as a preservative. There is a potential hazard that NaN3 reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water.
Preparation and Storage
Store at 2-8 degree C for up to one year. We recommend storing at –20 degree C for long-term storage. Avoid repeat freezing and thawing cycles
Other Notes
Small volumes of anti-DDX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DDX5 antibody
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-DDX5 antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-DDX5 antibody
WB: 0.2-2ug/ml
IP: 100-500ug/sample
ICC: 2-100 ug/ml

Quality Control of anti-DDX5 antibody
Western blot analysis of immunized recombinant protein, using anti-DDX5 monoclonal antibody.
anti-DDX5 antibody Quality Control image
Quality Control #2 of anti-DDX5 antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-DDX5 antibody Quality Control #2 image
Western Blot (WB) of anti-DDX5 antibody
Detection of DDX5 by Western blot.

Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa
anti-DDX5 antibody Western Blot (WB) (WB) image
Immunoprecipitation (IP) of anti-DDX5 antibody
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX5 mAb. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa
anti-DDX5 antibody Immunoprecipitation (IP) (IP) image
Immunocytochemistry (ICC) of anti-DDX5 antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX5 mAb. [Lot No. 2257C3a-1]
anti-DDX5 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for DDX5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60,563 Da
NCBI Official Full Name
Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), mRNA
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box helicase 5
NCBI Official Symbol
NCBI Official Synonym Symbols
p68; HLR1; G17P1; HUMP68
  [Similar Products]
NCBI Protein Information
probable ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); RNA helicase p68
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX5
UniProt Synonym Protein Names
DEAD box protein 5; RNA helicase p68
UniProt Gene Name
UniProt Entry Name
DDX5_HUMAN
NCBI Summary for DDX5
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for DDX5
DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: Nucleolus; EC 3.6.4.13; RNA-binding; RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity

Biological Process: nuclear mRNA splicing, via spliceosome; circadian rhythm; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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