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anti-DHFR antibody :: Mouse anti-Human DHFR Monoclonal Antibody

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Catalog # MBS200340
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

DHFR, Monoclonal Antibody

Popular Item
Full Product Name

DHFR antibody

Product Synonym Names
Dihydrofolate reductase; EC 1.5.1.3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
J00140 mRNA
3D Structure
ModBase 3D Structure for P00374
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5B2
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human DHFR (1-187aa) purified from E. coli
Clone Note
Anti-human DHFR mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DHFR protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-DHFR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DHFR antibody
Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
Applications Tested/Suitable for anti-DHFR antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-DHFR antibody
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate
anti-DHFR antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for DHFR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,672 Da
NCBI Official Full Name
dihydrofolate reductase isoform 1
NCBI Official Synonym Full Names
dihydrofolate reductase
NCBI Official Symbol
NCBI Official Synonym Symbols
DYR; DHFRP1
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NCBI Protein Information
dihydrofolate reductase
UniProt Protein Name
Dihydrofolate reductase
UniProt Gene Name
NCBI Summary for DHFR
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
UniProt Comments for DHFR
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Product References and Citations for anti-DHFR antibody
Chen MJ., et al. (1984) J Biol Chem. 259 (6): 3933-3943. ; Cody V., et al. (2009) Biochemistry. 48 (8): 1702-1711.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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