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anti-E2F6 antibody :: Mouse anti-Human E2F6 Monoclonal Antibody

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Catalog # MBS120109
Unit / Price
  0.1 mg  /  $345 +1 FREE 8GB USB
Product Name

E2F6, Monoclonal Antibody

Popular Item
Also Known As

Mouse monoclonal antibody Anti-Human E2F6

Product Synonym Names
Homo sapiens E2F transcription factor 6, transcript variant a, mRNA (cDNA clone MGC:15924 IMAGE:3535798), complete cds; E2F-6
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1826
OMIM
602944
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E2F6B9E2
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-E2F6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-E2F6 antibody
This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-E2F6 antibody
Dot Blot (DB), Immunocytochemistry (ICC)

Quality Control of anti-E2F6 antibody
Western blot analysis of immunized recombinant protein, using anti-E2F6 monoclonal antibody.
anti-E2F6 antibody Quality Control image
Quality Control #2 of anti-E2F6 antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-E2F6 antibody Quality Control #2 image
Immunocytochemistry (ICC) of anti-E2F6 antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-E2F6 mAb. [Lot No.E2F6B9E2-1]
anti-E2F6 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for E2F6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,103 Da
NCBI Official Full Name
Homo sapiens E2F transcription factor 6, mRNA
NCBI Official Synonym Full Names
E2F transcription factor 6
NCBI Official Symbol
NCBI Official Synonym Symbols
E2F-6
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NCBI Protein Information
transcription factor E2F6
UniProt Protein Name
Transcription factor E2F6
Protein Family
UniProt Gene Name
UniProt Entry Name
E2F6_HUMAN
NCBI Summary for E2F6
This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Comments for E2F6
E2F6: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase. Belongs to the E2F/DP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: transcription factor complex; nucleus

Molecular Function: DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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