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anti-EMD antibody :: Mouse Emerin Monoclonal Antibody

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Catalog # MBS4380434
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $340 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $340 +1 FREE 8GB USB
Product Name

Emerin (EMD), Monoclonal Antibody

Popular Item
Full Product Name

Emerin (Papillary Thyroid Carcinoma and EDMD Marker)

Product Synonym Names
EMD; Emerin; Emery Dreifuss muscular dystrophy (EDMD); STA
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50402
Clonality
Monoclonal
Isotype
IgG1
Clone Number
EMD/2167
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant human Emerin protein fragment (around aa 56-167)
Cellular Localization
Nuclear Membrane
Hu-chromosome Location
Xq28
Positive Control
K-562, HeLa or Jurkat cells. Kidney or Skin
Other Notes
Small volumes of anti-EMD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EMD antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-EMD antibody
IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Immunohistochemistry (IHC) of anti-EMD antibody
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).
anti-EMD antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-EMD antibody
Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).
anti-EMD antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-EMD antibody
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).
anti-EMD antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-EMD antibody
Western Blot Analysis of human HeLa Cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2167).
anti-EMD antibody Western Blot (WB) (WB) image
SDS-Page of anti-EMD antibody
SDS-PAGE Analysis Purified Emerin Mouse Monoclonal Antibody (EMD/2167).Confirmation of Integrity and Purity of Antibody.
anti-EMD antibody SDS-Page image
Testing Data of anti-EMD antibody
Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
anti-EMD antibody Testing Data image
NCBI/Uniprot data below describe general gene information for EMD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
37kDa
NCBI Official Full Name
emerin
NCBI Official Synonym Full Names
emerin
NCBI Official Symbol
NCBI Official Synonym Symbols
STA; EDMD; LEMD5
  [Similar Products]
NCBI Protein Information
emerin
UniProt Protein Name
Emerin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
EDMD; STA  [Similar Products]
NCBI Summary for EMD
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]
UniProt Comments for EMD
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
Product References and Citations for anti-EMD antibody
Bione, S., Maestrini, E., Rivella, S., Mancini, M., Regis, S., Romeo, G. and Toniolo, D. 1994. Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy. Nat. Genet. 8: 323-327.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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