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anti-EGFR antibody :: anti-Human Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control Monoclonal Antibody

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Catalog # MBS311326
Unit / Price
  0.1 mg  /  $710 +1 FREE 8GB USB
Testing Data
Product Name

Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control, Monoclonal Antibody

Also Known As

MAb to EGFR pS1047

Product Synonym Names
Monoclonal Antibody to Human Epidermal Growth Factor Receptor (EGFR), phosphorylated at Serine 1047
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
29
Chromosome Location
Chromosome: 7; NC_000007.13 (55086725..55275031). Location: 7p12
OMIM
131550
3D Structure
ModBase 3D Structure for P00533
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
1H9
Host
Host: Mouse
Source: Cell Culture
Species Reactivity
Human
Specificity
Epidermal Growth Factor Receptor (EGFR), phospho-Ser 1047 + Positive Control
The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the
Purity/Purification
Thiophilic adsorption and size exclusion chromatography
Form/Format
Purified, Lyophilized.
Reconstitute with 1ml water for 15 minutes at room temperature.
Concentration
100ug/ml (prior to lyophilization) (lot specific)
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Serine 1047
Affinity Constant
Not determined
Buffer
Lyophilized from 2X PBS, PEG and Sucrose
Preservative
0.09% Sodium Azide
Lyophilized
Yes
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at -20 degree C. After reconstitution, aliquot and store at -80 degree C. Thawed aliquots may be stored at 2 to 8 degree C for up to 3 months.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-EGFR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EGFR antibody
EIA/ELISA, Immunofluorescence Assay, Western Blot
Application Notes for anti-EGFR antibody
Immunoblotting: 0.5ug/ml for HRP/ECL detection.
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride
Immunocytochemistry: 0.1-1ug/ml<

Testing Data of anti-EGFR antibody
anti-EGFR antibody Testing Data image
NCBI/Uniprot data below describe general gene information for EGFR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
134,277 Da[Similar Products]
NCBI Official Full Name
epidermal growth factor receptor
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
  [Similar Products]
NCBI Protein Information
epidermal growth factor receptor; OTTHUMP00000159661; OTTHUMP00000159662; OTTHUMP00000159663; OTTHUMP00000209210; OTTHUMP00000209211; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Synonym Protein Names
Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
UniProt Gene Name
UniProt Synonym Gene Names
ERBB1  [Similar Products]
UniProt Entry Name
EGFR_HUMAN
NCBI Summary for EGFR
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for EGFR
Function: Cell surface receptor for EGF, but also for other members of the EGF family, such as TGF-alpha, BTC/betacellulin, AREGAREGB/amphiregulin, HBEGF, GP30 and vaccinia virus growth factor. Ligand binding triggers a conformation change, leading to activation of the kinase and subsequent phosphorylation of down-stream protein kinases. Is involved in the control of cell growth, proliferation and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65Isoform 2 may act as an antagonist of EGF action. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.56 Ref.59 Ref.60 Ref.61 Ref.62 Ref.63

Subunit structure: EGF binding triggers dimerization of the receptor and promotes autophosphorylation. The activate receptor dimer binds one EGF molecule. Heterodimer with ERBB2. Interacts with ERRFI1. Interaction with ERRFI1 inhibits dimerization of the kinase domain and autophosphorylation. Binds RIPK1. Interacts (via autophosphorylated C-terminal tail) with CBL. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts (autophosphorylated form) with PIK3C2B; the interaction may be indirect. Interacts with PELP1. Binds MUC1. Interacts with AP2M1. Interacts with GAB2. Interacts with COPG, upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Ref.26 Ref.28 Ref.31 Ref.32 Ref.33 Ref.37 Ref.41 Ref.44 Ref.52 Ref.54 Ref.55 Ref.60 Ref.62 Ref.63 Ref.64 Ref.65

Subcellular location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes. Ref.41 Ref.52 Ref.54 Ref.63Isoform 2: Secreted Ref.41 Ref.52 Ref.54 Ref.63.

Tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.40

Post-translational modification: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.30 Ref.34 Ref.35 Ref.36 Ref.38 Ref.39 Ref.42 Ref.43 Ref.45 Ref.46 Ref.47 Ref.48 Ref.50 Ref.51 Ref.59 Ref.60 Ref.62 Ref.63Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.

Involvement in disease: Defects in EGFR are associated with lung cancer (LNCR) [

MIM:211980].

Miscellaneous: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain.
Precautions
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Disclaimer
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