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anti-ESR2 antibody :: Mouse anti-Human ER-beta1 (Estrogen Receptor beta-1) Monoclonal Antibody

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Catalog # MBS439629
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

ER-beta1 (Estrogen Receptor beta-1) (ESR2), Monoclonal Antibody

Popular Item
Full Product Name

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Product Synonym Names
Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601663
3D Structure
ModBase 3D Structure for Q92731
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
ESR2/686
Host
Mouse
Species Reactivity
Human
Specificity
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Predominantly nuclear
Immunogen
C-terminus fragment of recombinant human estrogen receptor beta protein
Hu-Chromosome Location
14q23.2
Positive Control
MCF-7 Cells. Ovarian, Breast, Bladder, Gastric or Salivary carcinoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-ESR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ESR2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ESR2 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ESR2 antibody
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ESR2 antibody
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ESR2 antibody
Flow Cytometry of human ER beta on BT474 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunohistochemistry (IHC) of anti-ESR2 antibody
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ESR2 antibody
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC) of anti-ESR2 antibody
Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ESR2/686).
anti-ESR2 antibody Flow Cytometry (FC) (FC/FACS) image
SDS-PAGE of anti-ESR2 antibody
Purified ER-beta Mouse Monoclonal Antibody (ESR2/686). Confirmation of Integrity and Purity of Antibody.
anti-ESR2 antibody SDS-PAGE image
NCBI/Uniprot data below describe general gene information for ESR2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53-59 kDa
NCBI Official Full Name
estrogen receptor beta isoform 2
NCBI Official Synonym Full Names
estrogen receptor 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
  [Similar Products]
NCBI Protein Information
estrogen receptor beta
UniProt Protein Name
Estrogen receptor beta
UniProt Synonym Protein Names
Nuclear receptor subfamily 3 group A member 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
ESTRB; NR3A2; ER-beta  [Similar Products]
UniProt Entry Name
ESR2_HUMAN
NCBI Summary for ESR2
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for ESR2
ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: mitochondrion; nucleoplasm; nucleus

Molecular Function: DNA binding; enzyme binding; estrogen receptor activity; estrogen response element binding; protein binding; steroid binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity

Biological Process: cell-cell signaling; estrogen receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; signal transduction; transcription initiation from RNA polymerase II promoter
Product References and Citations for anti-ESR2 antibody
Skliris GP et. al. J Pathol 2002;197:155-62.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-ESR2 antibody
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